Determination of Fatty Acid Metabolism with Dynamic [11C]Palmitate Positron Emission Tomography of Mouse Heart In Vivo
Determination of Fatty Acid Metabolism with Dynamic [11C]Palmitate Positron Emission Tomography of Mouse Heart In Vivo
复制标题
DOI：
10.2310/7290.2015.00024
复制
发表时间：
2015-11-01
影响因子：
2.8
通讯作者：
Kundu, Bijoy K.
中科院分区：
文献类型：
--
作者：
Li, Yinlin;Huang, Tao;Kundu, Bijoy K.
本研究的目的是建立一种定量方法测量脂肪酸（FA）代谢的部分体积（PV）和溢出（SP）校正使用动态[C-11]棕榈酸正电子发射断层扫描（PET）图像的小鼠心脏在体内。使用Focus F-120 PET扫描仪对异氟烷麻醉下的4只18- 20周龄雄性C57 BL/6小鼠进行20分钟动态[C-11]棕榈酸酯PET扫描。模型校正的血液输入函数，通过该函数，在四室示踪动力学模型中，从小鼠心脏的动态[C-11]棕榈酸酯PET图像同时估计具有SP和PV校正的输入函数和代谢速率常数（k（1）-k（5）），用于确定心肌脂肪酸氧化（MFAO）、心肌FA酯化、心肌FA使用和心肌FA摄取的速率。因此在C57 BL/6小鼠中测量的MFAO为375.03 +/-43.83 nmol/min/g。这与离体灌注工作C57 BL/6小鼠心脏中测量的MFAO（约350 nmol/g/min和400 nmol/min/g）相当。FA代谢测定的第一次在小鼠心脏在体内使用动态[C-11]棕榈酸PET在四室示踪动力学模型。通过先前用小鼠心脏离体获得的结果验证了用该模型获得的MFAO。
The goal of this study was to establish a quantitative method for measuring fatty acid (FA) metabolism with partial volume (PV) and spill-over (SP) corrections using dynamic [C-11] palmitate positron emission tomographic (PET) images of mouse heart in vivo. Twenty-minute dynamic [C-11] palmitate PET scans of four 18-to 20-week-old male C57BL/6 mice under isoflurane anesthesia were performed using a Focus F-120 PET scanner. A model-corrected blood input function, bywhich the input function with SP and PV corrections and the metabolic rate constants (k(1)-k(5)) are simultaneously estimated from the dynamic [C-11] palmitate PET images of mouse hearts in a four-compartment tracer kinetic model, was used to determine rates of myocardial fatty acid oxidation (MFAO), myocardial FA esterification, myocardial FA use, and myocardial FA uptake. The MFAO thus measured in C57BL/6 mice was 375.03 +/- 43.83 nmol/min/g. This compares well to the MFAO measured in perfused working C57BL/6 mouse hearts ex vivo of about 350 nmol/g/min and 400 nmol/min/g. FA metabolism was measured for the first time in mouse heart in vivo using dynamic [C-11] palmitate PET in a four-compartment tracer kinetic model. MFAO obtained with this model was validated by results previously obtained with mouse hearts ex vivo.