Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries
Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries
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DOI：
10.1095/biolreprod60.2.381
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发表时间：
1999-02-01
影响因子：
3.6
通讯作者：
Sawyer, HR
中科院分区：
文献类型：
--
作者：
Bodensteiner, KJ;Clay, CM;Sawyer, HR
最近，转化生长因子β（TCF β）超家族的一个新成员，称为生长/分化因子-9（GDF-9），被证明在小鼠和人类的卵巢中表达，并且对于小鼠中超过初级（2型）卵泡阶段的正常卵泡发育至关重要。在本研究中，绵羊GDF-9基因的分离和鉴定，并在国内反刍动物卵巢GDF-9 mRNA的表达进行了研究。预测的绵羊GDF-9的氨基酸序列与人和小鼠GDF-9的同源性分别为77%和66%。使用同源S-35-反义探针的特异性杂交仅限于卵母细胞。与在小鼠中的类似研究（其中GDF-9首先在初级（2型）卵泡阶段开始检测到）相反，在绵羊和牛卵巢中，GDF-9 mRNA在原始（1型）卵泡阶段开始表达。所观察到的GDF-9在国内反刍动物卵母细胞中表达的时间和模式与GDF-9在这些物种中卵泡发生的启动和维持中的作用一致，并且支持卵泡生长和发育的早期阶段由卵巢内因子调节的一般概念。
Recently a novel member of the transforming growth factor beta (TCF beta) superfamily termed growth/differentiation factor-9 (GDF-9) was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is 77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous S-35-antisense probes was restricted to oocytes, In contrast to similar studies in mice in which GDF-9 was first detected beginning at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type 1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept that early stages of follicular growth and development are regulated by intraovarian factors.