Time-resolved fluorescence anisotropy studies on the interaction of biologically active polycations with phospholipid membranes
Time-resolved fluorescence anisotropy studies on the interaction of biologically active polycations with phospholipid membranes
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生物活性聚阳离子与磷脂膜相互作用的时间分辨荧光各向异性研究
DOI：
10.1016/0005-2736(90)90384-z
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
S. Tazuke
中科院分区：
文献类型：
--
作者：
T. Ikeda;Bong;H. Yamaguchi;S. Tazuke
本文用时间分辨荧光光谱法研究了高分子链内季铵盐与磷脂双层膜的相互作用。特别注意的是，聚阳离子诱导的流化的膜，这是很好地评价了时间分辨荧光各向异性测量的现象。基于锥内摆动模型分析了嵌在膜中的1，6-二苯基-1，3，5-己三烯（DPH）的荧光各向异性r（t）。在聚阳离子和带负电荷的磷脂酸膜之间观察到强烈的相互作用，如在将聚阳离子添加到酸性磷脂分散体中时残余极化值的大幅降低所证明的。这意味着DPH分子的圆锥摆动运动的锥角通过添加聚阳离子而增加，这表明酸性膜的聚阳离子诱导的流化。另一方面，嵌入在两性离子磷脂酰胆碱膜中的DPH的r（t）不受添加聚阳离子的显著影响，这可能是由于聚阳离子不与两性离子膜结合。我们的研究结果清楚地表明，聚阳离子消毒剂与带负电荷的膜强烈相互作用，诱导膜的流化。
Interaction of a polymeric in-chain quaternary ammonium salt with phospholipid bilayer membranes was studied by time-resolved fluorescence spectroscopy with the aid of a picosecond time-rcorrelated single photon counting system (instrument response function, 70 ps FWHM). Particular attention was paid to a phenomenon of polycation-induced fluidization of the membranes, which was well evaluated by time-resolved fluorescence anisotropy measurements. The fluorescence anisotropy,r(t), of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in the membranes was analyzed based on the wobbling-in-cone model. Strong interaction was observed between the polycation and a negatively charged phosphatidic acid membrane as demonstrated by a large decrease of residual polarization value on adding the polycation to the acidic phospholipid dispersion. This means that the cone angle of the wobbling-in-cone motion of the DPH molecule increases by the addition of the polycaton, indicative of the polycation-induced fluidization of the acidic membrane. On the other hand,r(t) of DPH embedded in a zwitterionic phosphatidylcholine membrane was not affected significantly by the addition of the polycation, which is presumably due to non-binding of the polycation to the zwitterionic membrane. Our results clearly indicate that the polycationic disinfectant interacts strongly with negatively charged membranes, inducing fluidization of the membranes.