Global distribution of nearly identical phage-encoded DNA sequences
Global distribution of nearly identical phage-encoded DNA sequences
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DOI：
10.1016/j.femsle.2004.05.042
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发表时间：
2004-07-15
影响因子：
2.1
通讯作者：
Rohwer, F
中科院分区：
文献类型：
--
作者：
Breitbart, M;Miyake, JH;Rohwer, F
噬菌体是地球上最丰富的生物实体，在生物地球化学循环、水平基因转移和确定微生物群落组成中起着重要作用。然而，人们对噬菌体多样性或噬菌体地理学知之甚少，而且还没有系统的努力来比较在不同生态系统中发现的噬菌体。在这里，我们报告说，T7样噬菌体DNA聚合酶序列存在于所研究的每个主要生物群中，包括海洋、淡水、沉积物、陆地、极端和后生动物相关的生物群。这些序列中的大多数属于一个独特的进化枝，这是唯一的远亲培养分离株。来自该进化枝的一些相同的T7样噬菌体编码的DNA聚合酶基因在多个不同环境中在核苷酸水平上>99%保守，这表明这些噬菌体在最近的进化时间内在生物群之间移动，并且T7样噬菌体的全球基因组库可能比以前假设的要小。(C)2004年，欧洲微生物学会联合会。Elsevier B.V.出版，保留所有权利。
Phages, the most abundant biological entities on the planet, play important roles in biogeochemical cycling, horizontal gene transfer, and defining microbial community composition. However, very little is known about phage diversity or biogeography, and there has not yet been a systematic effort to compare the phages found in different ecosystems. Here, we report that T7-like Podophage DNA polymerase sequences occur in every major biome investigated, including marine, freshwater, sediment, terrestrial, extreme, and metazoan-associated. The majority of these sequences belong to a unique clade that is only distantly related to cultured isolates. Some identical T7-like phage-encoded DNA polymerase genes from this clade were >99% conserved at the nucleotide level in multiple different environments, suggesting that these phages are moving between biomes in recent evolutionary time and that the global genomic pool for T7-like phages may be smaller than previously hypothesized. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.