Transcription and Recombination: When RNA Meets DNA
Transcription and Recombination: When RNA Meets DNA
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DOI：
10.1101/cshperspect.a016543
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发表时间：
2014-08-01
影响因子：
7.2
通讯作者：
Gaillard, Helene
中科院分区：
文献类型：
--
作者：
Aguilera, Andres;Gaillard, Helene
在细胞生理和增殖中，一种特别相关的现象是，通过转录可以强烈增强自发有丝分裂重组的事实。最接受的观点是，转录增加了通过重组修复的双链断裂和/或单链DNA间隙的发生。大多数休息将是由于转录对复制叉进展的影响，引起其停滞和/或破裂的影响。在这里，我们讨论了负责转录和重组之间交叉讨论的机制，重点是（1）转录复制冲突是重组DNA断裂的主要来源，以及（2）共转录R环作为主要的形成这种休息的原因。根据转录和复制之间的干扰以及RNA影响基因组动力学的方式，讨论了新的新兴问题和观点。
A particularly relevant phenomenon in cell physiology and proliferation is the fact that spontaneous mitotic recombination is strongly enhanced by transcription. The most accepted view is that transcription increases the occurrence of double-strand breaks and/or single-stranded DNA gaps that are repaired by recombination. Most breaks would arise as a consequence of the impact that transcription has on replication fork progression, provoking its stalling and/or breakage. Here, we discuss the mechanisms responsible for the cross talk between transcription and recombination, with emphasis on (1) the transcription-replication conflicts as the main source of recombinogenic DNA breaks, and (2) the formation of cotranscriptional R-loops as a major cause of such breaks. The new emerging questions and perspectives are discussed on the basis of the interference between transcription and replication, as well as the way RNA influences genome dynamics.