Biotransformation and bioconcentration of 6:2 and 8:2 polyfluoroalkyl phosphate diesters in common carp (Cyprinus carpio): Underestimated ecological risks
Biotransformation and bioconcentration of 6:2 and 8:2 polyfluoroalkyl phosphate diesters in common carp (Cyprinus carpio): Underestimated ecological risks
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鲤鱼（Cyprinus carpio）中 6:2 和 8:2 多氟烷基磷酸二酯的生物转化和生物富集：低估的生态风险
DOI：
10.1016/j.scitotenv.2018.11.297
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发表时间：
2019-03-15
影响因子：
9.8
通讯作者：
Yang, Liping
中科院分区：
文献类型：
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作者：
Chen, Meng;Guo, Tingting;Yang, Liping
多氟烷基磷酸酯（Polyfluoroalkyl phosphates esters，PAPs）广泛应用于各种商业产品中，并已在许多水生生物中检测到。本研究以6：2和8：2的diPAP水溶液对鲤鱼（Cyprinus carpio）进行染毒，研究其在鱼体内的生物积累和转化。在鲤鱼肝脏中鉴定出几种降解产物，包括氟调聚物不饱和羧酸（6：2和8：2 FTUCA）、5：3和7：3氟调聚物羧酸（5：3和7：3 FTCA）和全氟烷基羧酸（PFCAs）。此外，在鲤鱼胆汁中还检测到谷胱甘肽和葡萄糖醛酸结合物等II相代谢产物。8：2 diPAP显示出比6：2 diPAP更低的蓄积潜力，这可能是由于其相对较大的分子大小。然而，8：2 diPAP经历了更广泛的转化（转化率6.78-14.6 mol%），并且比6：2 diPAP（0.49-0.66 mol%）产生更多的I相代谢物。与肝S9组分的体外孵育证实，6：2和8：2的diPAP在鲤鱼肝中发生生物转化。酶活性的进一步分析表明，酸性磷酸酶（ACP）可能参与了鲤鱼6：2和8：2diPAP的I相代谢，而谷胱甘肽S-转移酶（GST）可能参与了鲤鱼6：2和8：2diPAP的II相代谢。(C)2018 Elsevier B. V.版权所有。
Polyfluoroalkyl phosphates esters (PAPs) are widely used in a variety of commercial products, and have been detected in many aquatic organisms. In this study, common carps (Cyprinus carpio) were administered with 6:2 and 8:2 diPAP in water to investigate their bio-accumulation and transformation in fish. Several degradation products, including fluorotelomer unsaturated carboxylic acids (6:2 and 8:2 FTUCA), 5:3 and 7:3 fluorotelomer carboxylic acids (5:3 and 7:3 FTCA), perfluoroalkyl carboxylates (PFCAs) were identified in the carp liver. In addition, several phase-II metabolites, such as glutathione- and glucuronide-conjugated compounds were detected in the carp bile. 8:2 diPAP displayed lower accumulation potential than 6:2 diPAP probably due to its relatively large molecular size. However, 8:2 diPAP experienced more extensive transformation (transformation rate 6.78-14.6 mol%) and produced more phase I metabolites than 6:2 diPAP (0.49-0.66 mol%). The in vitro incubation with the liver S9 fraction confirmed that biotransformation of 6:2 and 8:2 diPAP took place in the carp liver. Further analyses of enzyme activities indicated that acid phosphatase (ACP) could be involved in mediating phase I while glutathione S-transferase (GST) involved in phase II metabolism of 6:2 and 8:2 diPAP in carp. (C) 2018 Elsevier B.V. All rights reserved.