Green and Scalable Fabrication of High-Performance Biocatalysts Using Covalent Organic Frameworks as Enzyme Carriers
Green and Scalable Fabrication of High-Performance Biocatalysts Using Covalent Organic Frameworks as Enzyme Carriers
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DOI：
10.1002/anie.202208744
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发表时间：
2022-09-26
影响因子：
16.6
通讯作者：
Chen, Yao
中科院分区：
文献类型：
--
作者：
Zheng, Yunlong;Zhang, Sainan;Chen, Yao
酶的固定化对于各种关键的大规模生物催化过程的商业可行性是必不可少的。然而，固定化系统仍然存在挑战，例如负载大酶的困难、酶浸出以及大规模制造的限制。在此，我们描述了一种绿色和可扩展的策略，以制备高性能的生物催化剂，通过在原位组装酶与共价有机框架（COFs）在环境条件下（水溶液和室温）。所获得的生物催化剂具有优异的可重复使用性和稳定性，并且作为有效的生物催化剂用于不能被游离酶或传统酶固定化系统有效催化的重要工业反应。值得注意的是，这种通用的酶固定化平台适用于各种COF和酶。水溶液中的反应在短时间内发生（约20分钟）。10-30分钟），并可以很容易地按比例放大（约。2.3 g/反应）。
Enzyme immobilization is essential to the commercial viability of various critical large-scale biocatalytic processes. However, challenges remain for the immobilization systems, such as difficulties in loading large enzymes, enzyme leaching, and limitations for large-scale fabrication. Herein, we describe a green and scalable strategy to prepare high-performance biocatalysts through in situ assembly of enzymes with covalent organic frameworks (COFs) under ambient conditions (aqueous solution and room temperature). The obtained biocatalysts have exceptional reusability and stability and serve as efficient biocatalysts for important industrial reactions that cannot be efficiently catalyzed by free enzymes or traditional enzyme immobilization systems. Notably, this versatile enzyme immobilization platform is applicable to various COFs and enzymes. The reactions in an aqueous solution occurred within a short timeframe (ca. 10-30 min) and could be scaled up readily (ca. 2.3 g per reaction).