Evolution of transcription factors and the gene regulatory network in Escherichia coli
Evolution of transcription factors and the gene regulatory network in Escherichia coli
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DOI：
10.1093/nar/gkg210
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发表时间：
2003-02-15
影响因子：
14.9
通讯作者：
Teichmann, SA
中科院分区：
文献类型：
--
作者：
Babu, MM;Teichmann, SA
目前可获得的关于生物体转录调控网络的最详细的信息是关于原核生物大肠杆菌的信息。为了深入了解大肠杆菌调控网络的演变，我们分析了转录因子和调控基因的结构域和蛋白质家族的信息。在我们鉴定的271个转录因子中，大约有四分之三是双结构域蛋白，由一个DNA结合结构域沿着一个调节结构域组成。调节结构域主要结合小分子。许多转录因子具有相同的结构域结构，这意味着大约四分之三的转录因子是基因复制的结果。相反，几乎没有证据表明调控区与调控基因或转录因子与调控基因的复制。已知121种转录因子中有38种调控其他转录因子。这种放大效应，以及由转录因子直接调节的基因数量之间的巨大差异，意味着存在大约10个全局调节因子，每个调节因子控制的基因比其他转录因子多得多。
The most detailed information presently available for an organism's transcriptional regulation network is that for the prokaryote Escherichia coli. In order to gain insight into the evolution of the E.coli regulatory network, we analysed information obtainable for the domains and protein families of the transcription factors and regulated genes. About three-quarters of the 271 transcription factors we identified are two-domain proteins, consisting of a DNA-binding domain along with a regulatory domain. The regulatory domains mainly bind small molecules. Many groups of transcription factors have identical domain architectures, and this implies that roughly three-quarters of the transcription factors have arisen as a consequence of gene duplication. In contrast, there is little evidence of duplication of regulatory regions together with regulated genes or of transcription factors together with regulated genes. Thirty-eight, out of the 121 transcription factors for which one or more regulated genes are known, regulate other transcription factors. This amplification effect, as well as large differences between the numbers of genes directly regulated by transcription factors, means that there are about 10 global regulators which each control many more genes than the other transcription factors.