Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration
Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration
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DOI：
10.1007/bf00981886
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Dawes, Joan
中科院分区：
文献类型：
--
作者：
Bannon, Paul G.;Dean, Roger T.;Dawes, Joan
描述了一种用于分离和培养大量非粘附静止人外周血单核细胞用于粘附/迁移研究的方法。通过逆流离心淘洗分离具有gt 95%活力的Aprocalin 1.0 × 10-8细胞/白色细胞浓缩物。这些细胞最初通过分离程序活化，但在分离后48小时的最短培养时间和放射性标记后至少3小时的休息期后，常规地获得静止细胞，其具有低的基础粘附水平和对用佛波醇肉豆蔻酸酯乙酸酯活化的快速、可再现的超氧化物应答。
A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Aproximately 1.0 times 10-8 cells/white cell concentrate with gt 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiolabelling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.