Gig1, a novel antiviral effector involved in fish interferon response
Gig1, a novel antiviral effector involved in fish interferon response
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Gig1，一种参与鱼类干扰素反应的新型抗病毒效应物
DOI：
10.1016/j.virol.2013.10.029
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发表时间：
2014-01-05
期刊：
影响因子：
3.7
通讯作者：
Gui, Jian-Fang
中科院分区：
文献类型：
--
作者：
Sun, Fan;Zhang, Yi-Bing;Gui, Jian-Fang
脊椎动物干扰素(干扰素)通过诱导数百个干扰素刺激基因(ISGs)来防御病毒感染。大多数ISG在脊椎动物中都是保守的；然而，对物种特有的ISG知之甚少。本研究报道草鱼呼肠孤病毒(GCRV)诱导基因1(Gig1)是从紫外线灭活的GCRV感染的鲫鱼胚囊胚(CAB)细胞中筛选到的病毒诱导基因，是一种典型的鱼类ISG，它是由细胞内多聚(I：C)通过维甲酸诱导基因I(RIG-I)样受体触发的干扰素信号通路显著诱导的。瞬时或稳定过表达Gig1可以有效地阻止GCRV在鱼类细胞中的复制。令人惊讶的是，Gig1同源物仅在鱼类物种中发现，形成了一个新的基因家族。这些结果表明，存在一个鱼类特有的Gig1基因家族，该基因介导了一个新的鱼类干扰素抗病毒途径。(C)2013 Elsevier Inc.保留所有权利。
Vertebrate interferon (IFN) response defenses against viral infection through the induction of hundreds of IFN-stimulated genes (ISGs). Most ISGs are conserved across vertebrates; however, little is known about the species-specific ISGs. In this study, we reported that grass carp reovirus (GCRV)-induced gene 1 (Gig1), previously screened as a virus-induced gene from UV-inactivated GCRV-infected crucian carp (Carassius auratus) blastulae embryonic (CAB) cells, was a typical fish ISG, which was significantly induced by intracellular poly(I:C) through retinoic acid-inducible gene I (RIG-I)-like receptors-triggered IFN signaling pathway. Transient or stable overexpression of Gig1 prevented GCRV replication efficiently in cultured fish cells. Strikingly, Gig1 homologs were found exclusively in fish species forming a novel gene family. These results illustrate that there exists a Gig1 gene family unique to fish species and the founding gene mediates a novel fish IFN antiviral pathway. (C) 2013 Elsevier Inc. All rights reserved.