Separation and indirect detection of alkyl sulfonates and sulfates.
Separation and indirect detection of alkyl sulfonates and sulfates.
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烷基磺酸盐和硫酸盐的分离和间接检测。
DOI：
10.1093/chromsci/27.8.485
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发表时间：
1989
影响因子：
1.3
通讯作者：
Yuan,DX
中科院分区：
文献类型：
--
作者：
Pietrzyk,DJ;Rigas,PG;Yuan,DX
以铁(II)-1，10-邻菲咯啉，Fe(Phen)32+为流动相添加剂，在反相PRP-1上对烷基磺酸盐和硫酸盐进行了高效液相色谱分离。随着烷基链长的增加，保留率增加。对于给定的链长，烷基硫酸盐比相应的烷基磺酸盐保留得更多。影响保留的主要洗脱变量是流动相、溶剂和反阴离子浓度。间接光度检测用于在510 nm处检测Fe(Phen)32+盐吸收的烷基磺酸盐和硫酸盐。描述了多组分混合物的等度和梯度洗脱条件。根据分析物的不同，等浓度洗脱的检出限接近0.1nmol，梯度洗脱的检出限接近3nmol。
Iron(II) 1,10-phenanthroline, Fe(phen)32+, salts are used as mobile phase additives for the liquid chromatographic separation of alkyl sulfonates and sulfates on the reversed-phase PRP-1. As alkyl chain length increases retention increases. For a given chain length an alkyl sulfate is more retained than the corresponding alkyl sulfonate. Major elution variables that affect retention are mobile phase solvent and counteranion concentration. Indirect photometric detection is used to detect alkyl sulfonates and sulfates at 510 nm where Fe(phen)32+salts absorb. Conditions for isocratic and gradient elution of multicomponent mixtures are described. Detection limits depending on analyte approached 0.1 nmol for isocratic elution and 3 nmol for gradient elution.