Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis
Differential expression of senescence-associated mRNAs during leaf senescence induced by different senescence-inducing factors in Arabidopsis
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DOI：
10.1023/a:1005958300951
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发表时间：
1998-06-01
影响因子：
5.1
通讯作者：
Nam, HG
中科院分区：
文献类型：
--
作者：
Park, JH;Oh, SA;Nam, HG
4个cDNA克隆，命名为pSEN2，pSEN3，pSEN4，和pSEN5，在拟南芥叶片衰老过程中诱导的mRNA的特征。从在黑暗中孵育2天以加速衰老的离体叶片的cDNA文库中分离克隆，首先通过差异筛选，然后通过检查在年龄依赖性叶片衰老期间初步筛选的克隆的表达。这些cDNA克隆检测到的转录水平，因此，上调的年龄依赖性的方式，在黑暗诱导的叶片衰老。与此相反，当叶片衰老诱导乙烯，阿坝或茉莉酸甲酯，克隆检测到的转录水平差异调节衰老诱导激素依赖。在所有三种激素诱导的衰老过程中，pSEN4的转录物水平增加，而由pSEN2克隆检测到的转录物在乙烯诱导的衰老过程中没有增加。pSEN5的转录水平增加ABA诱导衰老，但在乙烯诱导衰老下降。pSEN3克隆检测到由这些因子差异调节的多个转录物。结果表明，尽管不同衰老诱导因子诱导的拟南芥叶片衰老症状相似，但不同衰老诱导因子诱导的叶片衰老过程中细胞的分子状态是不同的。pSEN3克隆编码多聚泛素，pSEN4克隆编码与内切木葡聚糖转移酶相关的肽。这一结果与衰老诱导基因在叶片衰老过程中的预期作用一致。
Four cDNA clones, named pSEN2, pSEN3, pSEN4, and pSEN5, for mRNAs induced during leaf senescence in Arabidopsis thaliana were characterized. The clones were isolated from a cDNA library of detached leaves incubated in darkness for 2 days to accelerate senescence, first by differential screening and then by examining expression of the primarily screened clones during age-dependent leaf senescence. Transcript levels detected by these cDNA clones, thus, were up-regulated in an age-dependent manner and during dark-induced leaf senescence. In contrast, when leaf senescence was induced by ethylene, ABA or methyljasmonate, the transcript level detected by the clones was differentially regulated depending on the senescence-inducing hormones. The transcript level for pSEN4 increased during senescence induced by all three hormones, while the transcript detected by the pSEN2 clone did not increase during senescence induced by ethylene. The transcript level for pSEN5 was increased upon ABA-induced senescence but decreased during ethylene-induced senescence. The pSEN3 clone detected multiple transcripts that are differentially regulated by these factors. The results show that, although the apparent senescence symptoms of Arabidopsis leaf appear similar regardless of the senescence-inducing factors, the detailed molecular state of leaf cells during senescence induced by different senescence-inducing factors is different. The pSEN3 clone encodes a polyubiquitin and the pSEN4 clone encodes a peptide related to endoxyloglucan transferase. This result is consistent with the expected roles of senescence-induced genes during leaf senescence.