Genomic constitution and variation in five partial amphiploids of wheat -: Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis
Genomic constitution and variation in five partial amphiploids of wheat -: Thinopyrum intermedium as revealed by GISH, multicolor GISH and seed storage protein analysis
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DOI：
10.1007/s00122-004-1720-y
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发表时间：
2004-09-01
影响因子：
5.4
通讯作者：
Liu, ZH
中科院分区：
文献类型：
--
作者：
Han, FP;Liu, B;Liu, ZH
利用基因组原位杂交（GISH）和双标记GISH（mcGISH）技术，对小麦与中间偃麦草（Thinopyrum intermedium）杂交获得的5个部分双倍体材料的细胞遗传学组成进行了研究。品系中1，2n=52，含有来自每个小麦基因组的14条染色体和10条Th。中间染色体，其中一对A-染色体组染色体具有Th。系中2（2n=54）具有完整的ABD小麦染色体组和12条Th染色体。中间染色体使用不同的荧光染料标记Th.以intermedium和各种二倍体小麦基因组DNA为探针，表明中1和中2都含有一对Th。中间染色体与小麦D染色体组具有显著的同源性。高分子量麦谷蛋白和醇溶蛋白分析表明，中1号和中2号具有完全相同的带型，包括所有小麦带和一条来自Th的特异高分子量带。中间的中1号和中2号具有较好的高分子量亚基育种价值。中3和中5，均为2n=56，不具有在GISH水平上可检测到的大体染色体畸变或易位。中4号染色体数为2n=56，含有完整的小麦ABD染色体和14条Th染色体。中间型染色体，有一对Th.中间染色体明显较小。多色GISH结果表明，中4还含有两对易位染色体，分别涉及A和D基因组。中3、中4和中5均含有一条强的麦醇溶蛋白带。中间的基于上述数据，可以得出结论，尽管这些新合成的部分双倍体具有类似二倍体的减数分裂行为和二体遗传，但它们发生了染色体片段的基因组间转移和/或序列渐渗。
Genomic in situ hybridization (GISH) and multicolor GISH (mcGISH) methodology were used to establish the cytogenetic constitution of five partial amphiploid lines obtained from wheat X Thinopyrum intermedium hybridizations. Line Zhong 1, 2n=52, contained 14 chromosomes from each of the wheat genomes plus ten Th. intermedium chromosomes, with one pair of A-genome chromosomes having a Th. intermedium chromosomal segment translocated to the short arm. Line Zhong 2, 2n=54, had intact ABD wheat genome chromosomes plus 12 Th. intermedium chromosomes. The multicolor GISH results, using different fluorochrome labeled Th. intermedium and the various diploid wheat genomic DNAs as probes, indicated that both Zhong 1 and Zhong 2 contained one pair of Th. intermedium chromosomes with a significant homology to the wheat D genome. High-molecular-weight (HMW) glutenin and gliadin analysis revealed that Zhong 1 and Zhong 2 had identical banding patterns that contained all of the wheat bands and a specific HMW band from Th. intermedium. Zhong 1 and Zhong 2 had good HMW subunits for wheat breeding. Zhong 3 and Zhong 5, both 2n=56, possessed no gross chromosomal aberrations or translocations that were detectable at the GISH level. Zhong 4 also had a chromosome number of 2n=56 and contained the complete wheat ABD-genome chromosomes plus 14 Th. intermedium chromosomes, with one pair of Th. intermedium chromosomes being markedly smaller. Multicolor GISH results indicated that Zhong 4 also contained two pairs of reciprocally translocated chromosomes involving the A and D genomes. Zhong 3, Zhong 4 and Zhong 5 contained a specific gliadin band from Th. intermedium. Based on the above data, it was concluded that inter-genomic transfer of chromosomal segments and/or sequence introgression had occurred in these newly synthesized partial amphiploids despite their diploid-like meiotic behavior and disomic inheritance.