Fine mapping of lcm1, a gene conferring chlorophyll-deficient golden leaf in Chinese cabbage (Brassica rapa ssp. pekinensis)
Fine mapping of lcm1, a gene conferring chlorophyll-deficient golden leaf in Chinese cabbage (Brassica rapa ssp. pekinensis)
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lcm1基因的精细定位，该基因赋予大白菜（Brassica rapa ssp. pekinensis）缺乏叶绿素的金黄色叶子
DOI：
10.1007/s11032-019-0945-z
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发表时间：
2019-04-01
影响因子：
3.1
通讯作者：
Liu, Zhiyong
中科院分区：
文献类型：
--
作者：
Fu, Wei;Ye, Xueling;Liu, Zhiyong
叶色突变体是研究光合色素代谢调控机制的宝贵资源。本研究通过甲烷磺酸乙酯(EMS)诱变，从大白菜野生型FT中筛选到一株大白菜叶绿素缺乏的金叶突变体Lcm1。金叶突变体lcm1的表型是叶片在整个发育过程中保持金色。色素测定表明，突变体lcm1的叶绿素含量低于野生型。突变体lcm1的叶绿体微结构中没有观察到明显的基粒片层。遗传分析表明，突变体lcm1表型受1对隐性核基因lcm1(BrChlH)控制。利用MutMap方法和Komcomptive等位基因特异性聚合酶链式反应(KomplativeAllele-SpecialPCR)对突变基因Lcm1的候选基因Bra006208编码镁螯合酶H亚基进行预测。Lcm1基因有5个外显子，第3外显子有一个单核苷酸替换(G到A)，导致氨基酸替换(A到V)。该SNP2523351位于LCM1(BrCHLH)蛋白高度保守的CobN-mg_chel结构域。本研究表明，lcm1基因在CHLH的功能中起着重要的作用，为进一步研究植物叶色的发育奠定了基础。
Leaf color mutants are valuable resources for studying regulatory mechanisms of photosynthetic pigment metabolism. In this study, a chlorophyll-deficient golden leaf mutant lcm1 of Chinese cabbage was identified from its wild-type FT by ethyl methanesulfonate (EMS) mutagenesis. The phenotype of the golden leaf mutant lcm1 was that the leaves remained golden throughout development. Pigment measurements showed that the chlorophyll content of the mutant lcm1 was less than that of the wild-type FT. There were no obvious grana lamellae observed in the chloroplast microstructure of the mutant lcm1. Genetic analysis revealed that the mutant lcm1 phenotype was controlled by a single recessive nuclear gene, lcm1 (BrChlH). The MutMap method and Kompetitive Allele Specific PCR genotyping were used to predict that Bra006208 encoding a Mg-chelatase H subunit, the candidate gene for the mutant lcm1. The 4249-bp lcm1 gene had five exons and a single nucleotide substitution (G to A) in the third exon that resulted in an amino acid substitution (A to V). This SNP2523351 occurred in the highly conserved CobN-Mg_chel domain of the LCM1 (BrCHLH) protein. This study suggests that lcm1 gene plays an important role in the function of CHLH and provides a solid foundation for further studies on the development of plant leaf color.