Infectivity test and serological relationships among various isolates of cucumber green mottle mosaic virus; some deduction of the invasion route of the virus into Japan.
Infectivity test and serological relationships among various isolates of cucumber green mottle mosaic virus; some deduction of the invasion route of the virus into Japan.
复制标题
黄瓜绿斑驳花叶病毒各分离株的感染性测定及血清学关系
DOI：
10.3186/jjphytopath.40.52
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
Y. Komuro
中科院分区：
文献类型：
--
作者：
H. Tochihara;Y. Komuro
为了推断黄瓜绿斑驳花叶病毒(CGMMV)侵入日本的可能途径，对日本CGMMV的3个分离株(Wa、Cu、Y)和英国的2个分离株(E1、E2)进行了感染性比较研究，琼脂双扩散法进行了两次血清学试验，以及吸收和未吸收的抗血清与同源和异源抗原的沉淀终点的相互关系。公鸡和一种来自印度的分离株 (C)，由 S.P. Raychaudhuri 提供。 TMV的番茄株(T)和普通株(OM)也被纳入血清学研究中。四个分离株； Wa、C、E1 和 E2 在传染性和血清学关系方面具有很强的相似性（表 1-3）。然而，沉淀素带显示出明显的刺激反应，表明以下分离株组合之间存在一些血清学差异； Wa-E1、Wa-E2、C-E1 和 C-E2（图 1）。 Wa在感染性和血清学测试中与C非常相似，这表明Wa和C实际上是相同的病毒株。葫芦种子经常从印度进口到日本，西瓜果实变质的情况与种子进口同时发生。据推测，佤族很可能是通过感染病毒的种子传入日本的。 Cu与这4个分离株的感染性和血清学关系存在显着差异，未发现与Cu相似的分离株。
To deduce the possible invasion route of cucumber green mottle mosaic virus (CGMMV) into Japan, comparative studies of infectivity and two serological tests by the agar gel double diffusion, and the reciprocal relations of precipitation end points of absorbed and unabsorbed antisera with homologous and heterologous antigens were made with three isolates of Japanese CGMMV (Wa, Cu, and Y), two isolates (E1 and E2) from England, supplied by L.J. Cock, and one isolate (C) from India, supplied by S.P. Raychaudhuri. Tomato strain (T) and ordinary strain (OM) of TMV were also included in serological studies.Four isolates; Wa, C, E1 and E2, had strong similarities in the infectivity and serological relationships with one another (Table 1-3). However, the precipitin bands showed a distinct spur reaction, indicating some serological differences between the following combination of the isolates; Wa-E1, Wa-E2, C-E1, and C-E2 (Fig. 1). Wa closely resembled C in the infectivity and serological tests, which suggested that Wa and C were virtually identical virus strain. Bottlegourd seeds have been imported frequently from India to Japan and occurrences of watermelon fruit's deterioration coincided with the importation of the seeds. It was deduced that Wa was most probably introduced to Japan by means of the seeds infected with the virus. Cu differed markedly in the infectivity and serological relationship from these four isolates, and isolates similar to Cu were not found.