Dynamic light scattering in protein crystallization droplets: Adaptations for analysis and optimization of crystallization processes
Dynamic light scattering in protein crystallization droplets: Adaptations for analysis and optimization of crystallization processes
复制标题
DOI：
10.1021/cg701067r
复制
发表时间：
2008-05-01
影响因子：
3.8
通讯作者：
Betzel, Christian
中科院分区：
文献类型：
--
作者：
Dierks, Karsten;Meyer, Arne;Betzel, Christian
研制了一种结合成像和动态光散射（DLS）技术的蛋白质结晶多孔板液滴常规测量系统。用几种标准蛋白对该系统进行了测试，发现该系统对于快速鉴定良好的结晶条件具有很高的价值。观察到蛋白质聚集体大小增加速率与晶体形成概率之间的关系。DLS是快速优化蛋白质结晶过程的合适工具。
A combined imaging and dynamic light scattering (DLS) system has been developed for routine measurements in droplets in the multiwell plates used in protein crystallization. The system was tested with several standard proteins and found to be of high value for rapid identification of good crystallization conditions. A relationship between the rate of protein-aggregate-size increase and the probability of crystal formation was observed. DLS is a suitable tool for a fast optimization of the protein crystallization process.