MicroRNAs of the mesothorax in Qinlingacris elaeodes, an alpine grasshopper showing a wing polymorphism with unilateral wing form
MicroRNAs of the mesothorax in Qinlingacris elaeodes, an alpine grasshopper showing a wing polymorphism with unilateral wing form
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DOI：
10.1017/s0007485315000991
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发表时间：
2015-12
影响因子：
1.9
通讯作者：
Ran Li;Guo-Fang Jiang;Qian Ren;Y. Wang;X.M. Zhou;C. F. Zhou;D. Z. Qin
中科院分区：
文献类型：
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作者：
Ran Li;Guo-Fang Jiang;Qian Ren;Y. Wang;X.M. Zhou;C. F. Zhou;D. Z. Qin
摘要微RNA（miRNAs）是目前公认的调控表型多样性的关键转录后调节因子。秦岭蝗（Qinlingacris elaeodes）是我国特有的高山蝗虫。成虫有三种翅型：无翅、单侧翅和短翅。由于其雌雄均为单侧翅型，是研究昆虫翅表型可塑性、发育和进化的理想物种。我们使用Illumina深度测序技术对从成年蝗虫的中胸制备的小RNA文库进行测序。产生了大约12，792，458个原始读段，其中854，580个高质量读段仅用于miRNA鉴定。在这项研究中，我们发现了49个保守的miRNAs，属于41个家庭和69个物种特异性的miRNAs。此外，7个miRNA * 的检测保守的miRNA和物种特异性的miRNA，这是支持基于聚合酶链反应的发夹形成前体。这是首次在高山蝗虫中描述miRNAs。本研究结果为进一步研究蝗虫miRNAs的分子调控和进化提供了参考。这些发现不仅丰富了昆虫miRNAs的研究内容，也为研究昆虫翅型的转录后调控奠定了基础。
Abstract MicroRNAs (miRNAs) are now recognized as key post-transcriptional regulators in regulation of phenotypic diversity. Qinlingacris elaeodes is a species of the alpine grasshopper, which is endemic to China. Adult individuals have three wing forms: wingless, unilateral-winged and short-winged. This is an ideal species to investigate the phenotypic plasticity, development and evolution of insect wings because of its case of unilateral wing form in both the sexes. We sequenced a small RNA library prepared from mesothoraxes of the adult grasshoppers using the Illumina deep sequencing technology. Approximately 12,792,458 raw reads were generated, of which the 854,580 high-quality reads were used only for miRNA identification. In this study, we identified 49 conserved miRNAs belonging to 41 families and 69 species-specific miRNAs. Moreover, seven miRNA*s were detected both for conserved miRNAs and species-specific miRNAs, which were supported by hairpin forming precursors based on polymerase chain reaction. This is the first description of miRNAs in alpine grasshoppers. The results provide a useful resource for further studies on molecular regulation and evolution of miRNAs in grasshoppers. These findings not only enrich the miRNAs for insects but also lay the groundwork for the study of post-transcriptional regulation of wing forms.