Wheat Brassinosteroid-Insensitive1 (TaBRI1) Interacts with Members of TaSERK Gene Family and Cause Early Flowering and Seed Yield Enhancement in Arabidopsis.
Wheat Brassinosteroid-Insensitive1 (TaBRI1) Interacts with Members of TaSERK Gene Family and Cause Early Flowering and Seed Yield Enhancement in Arabidopsis.
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DOI：
10.1371/journal.pone.0153273
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Khurana P
中科院分区：
文献类型：
--
作者：
Singh A;Breja P;Khurana JP;Khurana P
油菜素类固醇(BRS)激素对植物的生长发育和免疫反应具有重要作用。当它们与胞外富含亮氨酸重复序列(LRR)结构域结合时，它们被跨膜受体激酶油菜素类固醇不敏感1(BRI1)感知。我们从普通小麦中克隆和鉴定了TaBRI1基因，并在拟南芥中建立了过表达的转基因基因，以破译其功能。TaBRI1蛋白由一个信号肽、25个富含亮氨酸的重复序列(LRR)、一个跨膜区和一个C-末端的激酶区组成。分析确定了TaBRI1与小麦体细胞胚胎发生受体激酶(TaSERKs)基因家族的5个成员(TaSERK1、TaSERK2、TaSERK3、TaSERK4和TaSERK5)在质膜上的相互作用。此外，TaBRI1基因在拟南芥中的过表达导致了拟南芥提前开花、角果大小和种子产量的增加。对TaBRI1高表达植株的根生长分析表明，转基因植株对表油菜素内酯(epi-BL)激素高度敏感，且呈剂量依赖关系。有趣的是，从叶绿素含量、光系统II活性和膜稳定性来看，转基因拟南芥植株在苗期表现出耐热表型。基于微阵列分析的转录组图谱显示，与油菜素类固醇信号通路、非生物胁迫反应、防御反应和转录因子相关的几个基因表达上调。这些研究预测了TaBRI1基因在植物生长发育中的可能作用，赋予植物对热胁迫的耐受性。
Brassinosteroids (BRs) hormones are important for plant growth, development and immune responses. They are sensed by the transmembrane receptor kinase Brassinosteroid-Insensitive 1 (BRI1) when they bind to its extracellular Leu-rich repeat (LRR) domain. We cloned and characterized the TaBRI1 from T. aestivum and raised overexpression transgenics in Arabidopsis to decipher its functional role. TaBRI1 protein consists of a putative signal peptide followed by 25 leucine rich repeats (LRR), a transmembrane domain and a C-terminal kinase domain. The analysis determined the interaction of TaBRI1 with five members of the wheat Somatic Embryogenesis Receptor Kinase (TaSERKs) gene family (TaSERK1, TaSERK2, TaSERK3, TaSERK4 and TaSERK5), at the plasma membrane. Furthermore, overexpression of TaBRI1 in Arabidopsis leads to the early flowering, increased silique size and seed yield. Root growth analysis of TaBRI1 overexpressing transgenic plants showed hypersensitivity to epi-brassinolide (epi-BL) hormone in a dose-dependent manner. Interestingly, transgenic Arabidopsis plants show thermotolerance phenotype at the seedling stages as revealed by chlorophyll content, photosystem II activity and membrane stability. The transcriptome profiling on the basis of microarray analysis indicates up-regulation of several genes related to brassinosteroid signaling pathway, abiotic stress response, defense response and transcription factors. These studies predict the possible role of TaBRI1 gene in plant growth and development imparting tolerance to thermal stress.