Large-scale imaging of cortical network activity with calcium indicators

Large-scale imaging of cortical network activity with calcium indicators
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DOI:
10.1016/j.neures.2005.02.004
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发表时间:
2005-06-01
影响因子:
2.9
通讯作者:
Yuste, R
Yuste, R
中科院分区:
医学4区
文献类型:
--
作者:
Ikegaya, Y;Le Bon-Jego, M;Yuste, R

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大量装载钙指标提供了一个独特的机会,重建活动的皮质网络与单细胞分辨率。在这里,我们描述了详细的方法,批量加载的AM染料,我们开发的,并已改善与旋转盘共聚焦显微镜成像。(c)2005年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Bulk loading of calcium indicators has provided a unique opportunity to reconstruct the activity of cortical networks with single-cell resolution. Here we describe the detailed methods of bulk loading of AM dyes we developed and have been improving for imaging with a spinning disk confocal microscope. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.