Effect of grape seed extract on maternal toxicity and in utero development in mice treated with zearalenone
Effect of grape seed extract on maternal toxicity and in utero development in mice treated with zearalenone
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DOI：
10.1007/s11356-018-4011-x
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发表时间：
2019-02-01
影响因子：
5.8
通讯作者：
Abdel-Wahhab, Mosaad A.
中科院分区：
文献类型：
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作者：
Althali, Nouf J.;Hassan, Aziza M.;Abdel-Wahhab, Mosaad A.
本研究的目的是测定葡萄籽提取物(GSE)中多酚的含量，并评价其对玉米赤霉烯酮(Zen)所致母体毒性和小鼠宫内发育缺陷的保护作用。妊娠6~13d的小鼠分为5组：对照组、玉米油(0.1ml/只)处理组、ZEN处理组(25 mg/kg bw)、GSE处理组(150 mg/kg bw)和Zen+GSE处理组。于妊娠第19天处死动物，采集骨髓标本进行微核试验。进行母体毒性和发育毒性试验。高效液相色谱分析表明，GES富含没食子酸、丁香酸、香草醛、槲皮素和香豆酸。Zen对母鼠有严重的母体毒性和发育毒性，表现为骨髓微核形成增加，母鼠增重和窝重下降。还可导致胎儿生长迟缓，流产、吸收胎儿增多，胎儿骨化异常，胎儿血肿增多。GSE对妊娠小鼠和发育中的胎儿有积极的作用。此外，它还抵消了禅宗对水坝和胎儿的有害影响。可以得出的结论是，在高度流行的地区，GSE中的多酚是一种很有希望的候选物质，可以预防Zen毒性。
The aims of this study were to determine the polyphone content of grape seed extract (GSE) and to assess their protective effects against zearalenone (ZEN)-induced maternal toxicity and in utero development defects in mice. Five groups of pregnant mice were treated orally during days 6-13 of gestation as follows: control group, corn oil as vehicle (0.1ml/mice)-treated group, ZEN-treated group (25mg/kg b.w), GSE-treated group (150mg/kg b.w.), and ZEN plus GSE-treated group. All animals were sacrificed on the 19th day of gestation and samples of bone marrow were collected for the micronucleus assay. The maternal and developmental toxicity were carried out. The HPLC analyses revealed that GES is rich in gallic acid, syringic acid, vanillin, quercetin, and coumaric acid. ZEN administration resulted in severe maternal and developmental toxicity which included an increase of micronuclei formation in bone marrow, decreased maternal weight gain, and litter weight. It also induces fetal growth retardation, increased number of the aborted dams and resorbed fetuses, abnormality of fetal bone ossification, and number of fetuses with a hematoma. GSE showed positive effects on the pregnant mice and the developing fetuses. Moreover, it counteracted the detrimental effects of ZEN in dams and fetuses. It could be concluded that polyphenols in GSE are a promising candidate to protect against ZEN toxicity in highly endemic areas.