A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae) - Drosophila melanogaster interaction
A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae) - Drosophila melanogaster interaction
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DOI：
10.1016/j.ibmb.2004.10.004
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发表时间：
2005-02-01
影响因子：
3.8
通讯作者：
Poirie, M
中科院分区：
文献类型：
--
作者：
Labrosse, C;Staslak, K;Poirie, M
为了保护它的卵。内寄生蜂 Leptopilina boulardi 将免疫抑制因子注入果蝇宿主幼虫中。这些因素位于雌性长腺和储库中。我们分析了这些组织的蛋白质含量，发现有毒力和无毒力寄生蜂菌株之间的蛋白质含量存在很大差异。洗脱了有毒长腺体中存在的四种蛋白质条带，并在体内评估了它们的免疫抑制作用，从而证明了其中一种蛋白质的主要作用。相应的 cDNA 编码预测的 30 kDa 亚基，其中包含 Ras 同源 GTP 酶激活蛋白 (RhoGAP) 结构域，表明可能参与肌动蛋白细胞骨架变化的调节。使用蛋白质印迹实验，我们发现这种蛋白质在有毒雌性长腺体中含量丰富，但在失去长腺体的有毒雌性或无毒黄蜂的长腺体中检测不到。讨论了它在改变参与果蝇细胞免疫反应的宿主板层细胞的形态和粘附特性方面的潜在作用。 (C) 2004 Elsevier Ltd. 保留所有权利。
To protect its eggs. the endoparasitoid wasp Leptopilina boulardi injects immune suppressive factors into Drosophila melanogaster host larvae. These factors are localized in the female long gland and reservoir. We analyzed the protein content of these tissues and found that it strongly differed between virulent and avirulent parasitoid strains. Four protein bands present in virulent long,glands were eluted and their immune suppressive effect was assessed in vivo, allowing demonstrating a major effect of one of these proteins. The corresponding cDNA encodes a predicted 30 kDa Subunit containing a Ras homologous GTPase Activating Protein (RhoGAP) domain, suggesting a possible involvement in the regulation of actin cytoskeleton changes. Using Western-blot experiments, we showed that this protein is abundant ill Virulent female long glands but is undetectable in virulent females deprived of long glands or in long glands from avirulent wasps. Its potential role in modifying the morphology and the adhesive properties of the host lamellocytes involved in Drosophila cellular immune responses, is discussed. (C) 2004 Elsevier Ltd. All rights reserved.