BOVINE GALLBLADDER MUCIN ACCELERATES CHOLESTEROL MONOHYDRATE CRYSTAL-GROWTH IN MODEL BILE
BOVINE GALLBLADDER MUCIN ACCELERATES CHOLESTEROL MONOHYDRATE CRYSTAL-GROWTH IN MODEL BILE
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DOI：
10.1016/0016-5085(93)90364-i
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发表时间：
1993-05-01
期刊：
影响因子：
29.4
通讯作者：
SMITH, BF
中科院分区：
文献类型：
--
作者：
AFDHAL, NH;NIU, N;SMITH, BF
背景：胆囊粘蛋白加速胆固醇晶体成核，这是胆结石发病的早期步骤。为了研究胆囊粘蛋白在核化后胆结石成熟中的作用，粘蛋白对胆固醇一水合物晶体生长的影响进行了研究，在一个新的model system.Methods：胆固醇晶体的均匀大小在模型胆汁中孵育在37 °C与不同的胆固醇饱和指数。晶体大小通过测量偏振光显微镜下的单个晶体的宽度和长度，并计算平均晶体area.Results：晶体生长依赖于胆汁中胆固醇过饱和的程度。牛胆囊粘蛋白（0.5-8 mg/mL）以浓度和时间依赖的方式促进过饱和模型胆汁中晶体的生长，与单独的牛血清白蛋白或模型胆汁相比（P< 0.05）。胆固醇晶体生长伴随着胆固醇饱和度的逐渐降低和总胆固醇晶体质量的增加。晶体生长也伴随着总晶体数的减少，这表明净转移的胆固醇到更大的crystals.Conclusions：加速胆固醇晶体生长的胆囊粘蛋白可能是胆固醇结石的核后成熟的病理生理重要性。
Background:Gallbladder mucin accelerates cholesterol crystal nucleation, an early step in the pathogenesis of gallstones. To examine the role of gallbladder mucin in postnucleation gallstone maturation, the influence of mucin on cholesterol monohydrate crystal growth was studied in a novel model system.Methods:Cholesterol crystals of a uniform size were incubated in model biles at 37 °C with varying cholesterol saturation indices. Crystal size was quantitated by measuring the width and length of individual crystals under polarizing light microscopy and calculating average crystal area.Results:Crystal growth was dependent on the degree of cholesterol supersaturation of bile. Bovine gallbladder mucin (0.5–8 mg/mL) accelerated crystal growth in supersaturated model bile in a concentration- and time-dependent fashion compared with control incubations with bovine serum albumin or model bile alone (P< 0.05). Cholesterol crystal growth was accompanied by a progressive decrease in cholesterol saturation and an increase in total cholesterol crystal mass. Crystal growth was also accompanied by a decrease in total crystal number, suggesting net transfer of cholesterol to larger crystals.Conclusions:The acceleration of cholesterol crystal growth by gallbladder mucin may be of pathophysiological importance in the postnucleation maturation of cholesterol gallstones.