HUMIC-ACID VITAMIN AGAR, A NEW MEDIUM FOR THE SELECTIVE ISOLATION OF SOIL ACTINOMYCETES
HUMIC-ACID VITAMIN AGAR, A NEW MEDIUM FOR THE SELECTIVE ISOLATION OF SOIL ACTINOMYCETES
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DOI：
10.1016/0385-6380(87)90108-7
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发表时间：
1987-10-01
期刊：
影响因子：
--
通讯作者：
NONOMURA, H
中科院分区：
文献类型：
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作者：
HAYAKAWA, M;NONOMURA, H
开发了一种新的培养基，命名为HV琼脂，含有土壤腐殖酸作为唯一的碳源和氮源。HV琼脂用于土壤放线菌的分离和计数上级目前使用的其他培养基，包括胶体几丁质琼脂、甘油-精氨酸琼脂和淀粉-硝酸盐琼脂：它允许属于链霉菌属、小单孢菌属、小双孢菌属、链孢子囊菌属、诺卡氏菌属、达氏孢子囊菌属、小四孢菌属和热单孢菌属的每一属的最大数量的放线菌菌落在平板上生长，同时限制真细菌的生长HV琼脂支持这些放线菌的充分生长和良好的产孢。即使当孢子悬浮液被用作接种物，HV琼脂产生了显着更大的放线菌，特别是属小单孢菌，小双孢菌，链孢子囊菌，Dacquillussporangium和Streptomonospora的菌株，比甘油-精氨酸琼脂。发现这些放线菌的孢子在20 ℃处理后萌发时被活化。在温育之前，将其与0.2%的腐殖酸溶液在室温下孵育30分钟。
A new medium, designated HV agar, containing soil humic acid as the sole source of carbon and nitrogen was developed. The HV agar was superior to other currently used media, including colloidal chitin agar, glycerol-arginine agar and starch-nitrate agar, for the isolation and enumeration of soil actinomycetes: It allowed the growth of the largest numbers of actinomycete colonies belonging to each genus of Streptomyces, Micromonospora, Microbispora, Streptosporangium, Nocardia, Dactylosporangium, Microtetraspora and Thermomonospora on the plate, while restricting the development of true bacteria. The HV agar supported adequate growth and good sporulation for these actinomycetes. Even when spore suspensions were used as the inoculum, the HV agar produced remarkably larger numbers of actinomycetes, especially strains of the genera Micromonospora, Microbispora, Streptosporangium, Dactylosporangium and Saccharomonospora, than did glycerol-arginine agar. It was found that the spores of these actinomycetes were activated upon germination by treatment at 20.degree. C for 30 min with a 0.2% solution of humic acid prior to incubation.