Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis
Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis
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DOI：
10.1038/labinvest.3700452
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发表时间：
2006-09-01
影响因子：
5
通讯作者：
Kaga, Kimitaka
中科院分区：
文献类型：
--
作者：
Nakaya, Muneo;Dohi, Makoto;Kaga, Kimitaka
到目前为止，还没有一种生理学方法来评估小鼠的鼻过敏反应。增强停顿(Penh)已被用作反映下呼吸道变化的指标。然而，最近，对于Penh系统的意义存在分歧；这是因为Penh本质上不是一个生理参数，它可能不一定代表下呼吸道的变化。本研究的目的是探讨Penh是否可用于分析小鼠的鼻部超敏反应。BALB/c小鼠在清醒状态下，以卵清蛋白(OVA)为致敏物质，每日一次鼻腔注射致敏。在致敏过程中的每个时间点测量Penh，或在最后一次鼻腔激发后跟踪Penh值的一系列变化，并评估治疗效果。在致敏和鼻腔激发后，Penh值逐渐升高，在第14天时有显著差异。IgE、鼻粘膜嗜酸性粒细胞浸润和OVA诱导的症状的变化都与Penh的升高密切相关。在OVA鼻腔刺激后第19天，Penh逐渐升高，并在激发后25min达到最大值。地塞米松或组胺H1阻滞剂可显著抑制Pen H的这种增加。通过测量气道阻力和支气管肺泡灌洗液，并通过组织学检查，我们证实了鼻腔内OVA激发并没有引起支气管收缩。这些结果清楚地表明，Penh可以作为研究鼻部超敏反应的有用的非侵入性指标。
Until now there has been no method for physiologically evaluating nasal hypersensitivity in mice. Enhanced pause ( Penh) has been used as an indicator that reflects changes in the lower airway. Recently, however, there is disagreement regarding the significance of the Penh system; this is because Penh is not essentially a physiological parameter, and it might not necessarily represent a change in the lower respiratory tract. The purpose of the present study is to investigate whether Penh could be applicable for analyzing nasal hypersensitivity in mice. BALB/c mice were sensitized with ovalbumin ( OVA) through a combination of intraperitoneal injection and daily intranasal challenge in an awake condition. Penh was measured at each time point during sensitization, or a serial change in Penh value was followed after the final nasal challenge and the effect of treatment was assessed. Following sensitization and nasal challenge, the Penh value gradually increased and showed a significant difference on day 14. Changes in IgE, eosinophil infiltration into nasal mucosa, and OVA-induced symptoms all strongly correlated with the increase in Penh. On day 19, after OVA nasal provocation, Penh gradually increased and reached maximal values 25 min after the challenge. Pretreatment with dexamethasone or a histamine H1 blocker significantly suppressed this increase in Penh. We confirmed that intranasal OVA challenge did not induce bronchoconstriction by measuring airway resistance and bronchoalveolar lavage fluid, and through histological examination. These results clearly demonstrate that Penh could be a useful noninvasive indicator for studying nasal hypersensitivity.