WFL, a wheat FLORICAULA/LEAFY ortholog, is associated with spikelet formation as lateral branch of the inflorescence meristem.
WFL, a wheat FLORICAULA/LEAFY ortholog, is associated with spikelet formation as lateral branch of the inflorescence meristem.
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DOI：
10.1266/ggs.81.13
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发表时间：
2006-02
影响因子：
1.1
通讯作者：
N. Shitsukawa;Aiko Takagishi;Chihiro Ikari;S. Takumi;K. Murai
中科院分区：
文献类型：
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作者：
N. Shitsukawa;Aiko Takagishi;Chihiro Ikari;S. Takumi;K. Murai
金鱼草属植物的FLORICALIA（FLO）和拟南芥属植物的LEAFY（LFY）编码植物特异性转录因子，这些转录因子是确定花分生组织身份所必需的。我们分离了小麦FLO/LFY同源基因WFL，并分析了其表达模式。RT-PCR分析表明，WFL主要在幼穗中表达。利用原位杂交技术对WFL在生殖发育过程中的表达模式进行了详细的分析。除了小穗起始部位为腋生分生组织外，在幼穗各层中均观察到WFL转录本。在双棱期，WFL转录本定位于下棱，但在小穗分生组织起始的上棱缺失。WFL的表达模式表明，WFL是与小穗的形成，而不是花分生组织的身份在小麦。随着小花发育的进行，WFL转录本在发育中的内稃中检测到，但在其他花器官中检测不到，这表明WFL可能在小麦小花的内稃发育中发挥新的作用。
FLORICAULA (FLO) of Antirrhinum and LEAFY (LFY) of Arabidopsis encode plant-specific transcription factors, which are necessary and sufficient to specify floral meristem identity. We isolated WFL, a wheat FLO/LFY ortholog, and analyzed its expression pattern. RT-PCR analysis indicated that WFL is expressed predominantly in young spike. The WFL expression pattern during reproductive development was analyzed in more detail by using in situ hybridization technique. WFL transcripts were observed in all layers of the young spike excepting spikelet initiation sites as axillary meristem. In the double-ridge stage, WFL transcripts were localized in the lower ridge but were absent in the upper ridge, where spikelet meristem initiates. The WFL expression pattern indicated that WFL is associated with spikelet formation rather than floral meristem identity in wheat. As development of floret proceeds, the WFL transcripts were detectable in the developing palea, but not in other floral organs, suggesting that WFL may play a novel role in developing palea in the wheat floret.