Microbial physiology-based model of ethanol metabolism in subsurface sediments
Microbial physiology-based model of ethanol metabolism in subsurface sediments
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DOI：
10.1016/j.jconhyd.2011.04.002
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发表时间：
2011-07-01
影响因子：
3.6
通讯作者：
Roden, Eric E.
中科院分区：
文献类型：
--
作者：
Jin, Qusheng;Roden, Eric E.
建立了一个基于微生物生理学的生物地球化学反应模型，用于模拟乙醇代谢及其对缺氧地下环境化学的影响。该模型考虑了降解乙醇的潜在微生物代谢作用，包括通过减少不同的电子受体直接或合成氧化乙醇的代谢作用。在潜在的新陈代谢中，那些在环境中活跃的新陈代谢可以通过将模型与实验观察相匹配来推断。这一方法被应用于田纳西州橡树岭美国能源部现场研究中心的一项批量沉淀物泥浆实验，该实验研究了来自区域2的受铀污染的含水层沉积物中的乙醇代谢。根据模拟结果，乙醇的反硝化完全氧化、铁还原乙醇的不完全氧化、乙醇发酵生成乙酸酯和H-2、氢营养硫酸盐还原和乙酰碎屑产甲烷作用均对含水层沉积物中乙醇的降解有显著贡献。活性代谢物的组合为探索乙醇修饰如何影响环境化学，包括铀的存在和水平提供了一个框架。这些结果也可用于探索不同的微生物代谢如何影响生物修复策略的进展和效果。(C)2011爱思唯尔B.V.保留所有权利。
A biogeochemical reaction model was developed based on microbial physiology to simulate ethanol metabolism and its influence on the chemistry of anoxic subsurface environments. The model accounts for potential microbial metabolisms that degrade ethanol, including those that oxidize ethanol directly or syntrophically by reducing different electron acceptors. Out of the potential metabolisms, those that are active in the environment can be inferred by fitting the model to experimental observations. This approach was applied to a batch sediment slurry experiment that examined ethanol metabolism in uranium-contaminated aquifer sediments from Area 2 at the U.S. Department of Energy Field Research Center in Oak Ridge, TN. According to the simulation results, complete ethanol oxidation by denitrification, incomplete ethanol oxidation by ferric iron reduction, ethanol fermentation to acetate and H-2, hydrogenotrophic sulfate reduction, and acetoclastic methanogenesis: all contributed significantly to the degradation of ethanol in the aquifer sediments. The assemblage of the active metabolisms provides a frame work to explore how ethanol amendment impacts the chemistry of the environment, including the occurrence and levels of uranium. The results can also be applied to explore how diverse microbial metabolisms impact the progress and efficacy of bioremediation strategies. (C) 2011 Elsevier B.V. All rights reserved.