Modeling success and failure of Langmuir-Blodgett transfer of phospholipid bilayers to silicon dioxide.
Modeling success and failure of Langmuir-Blodgett transfer of phospholipid bilayers to silicon dioxide.
复制标题
模拟磷脂双层到二氧化硅的 Langmuir-Blodgett 转移的成功和失败。
DOI：
10.1016/s0006-3495(95)80309-7
复制
发表时间：
1995
影响因子：
3.4
通讯作者：
Yager,P
中科院分区：
文献类型：
--
作者：
Osborn,TD;Yager,P
在固体和多孔基底上通过从空气-水界面的单层的Langmuir-Blodgett转移形成平面磷脂双分子层可能具有更大的实用性，如果该过程不是不可再现的和知之甚少的话。为此，能量转移两个磷脂单层的亲水性表面已被检查。一个近似的数学关系，制定有关的前体单层的表面压力的双层内产生的张力。数据表明，双层转移可以重复进行，即使与难治性磷脂，如磷脂酰胆碱，但只有在一个非常窄的范围内的前体单层表面压力。该范围与双层的裂解张力有关。通过荧光显微镜检查的表面压力的成功范围内和以下形成的膜的形态，和观察到的功能进行了讨论，在上面的关系。这些结果提供了实用的指导方针，成功地形成脂质双层亲水表面上，这些准则应证明是有用的生物膜的性质的研究和开发的双层生物传感器。
Formation of planar phospholipid bilayers on solid and porous substrates by Langmuir-Blodgett transfer of monolayers from the air-water interface could be of much greater utility if the process were not irreproducible and poorly understood. To that end the energetics of transferring two phospholipid monolayers to a hydrophilic surface has been examined. An approximate mathematical relationship is formulated that relates the surface pressure of the precursor monolayers to the tension within the bilayer created. Data are presented that demonstrate that bilayer transfer can be carried out reproducibly even with refractory phospholipids such as phosphatidylcholine, but only over a very narrow range of precursor monolayer surface pressures. This range is related to the lysis tension of the bilayer. The morphology of films formed within and below the successful range of surface pressures are examined by fluorescence microscopy, and the observed features are discussed in terms of the relationship above. These results provide practical guidelines for successful formation of lipid bilayers on hydrophilic surfaces; these guidelines should prove useful for research into the properties of biomembranes and for development of bilayer-based biosensors.