Immunoreactivity against tau, amyloid precursor protein, and beta-amyloid in the human retina.
Immunoreactivity against tau, amyloid precursor protein, and beta-amyloid in the human retina.
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发表时间：
1995
影响因子：
4.4
通讯作者：
K. Löffler;D. Edward;M. Tso
中科院分区：
文献类型：
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作者：
K. Löffler;D. Edward;M. Tso
大脑中β -淀粉样蛋白和淀粉样蛋白相关蛋白tau和淀粉样蛋白前体蛋白（APP）的免疫反应性（IR）增加与阿尔茨海默病等神经退行性疾病的发病机制有关。然而，这些蛋白的表达尚未在正常或患病的人视网膜中进行研究。方法采用免疫组织化学技术，研究不同年龄（n = 24）、视网膜色素变性（RP, n = 6）和年龄相关性黄斑变性（ARMD, n = 10）患者视网膜中抗-tau-1、抗-tau-2、抗- app和抗- β -淀粉样蛋白IR的分布和年龄相关性变化。结果Tau-1免疫反应性在视网膜内层强烈，不随年龄或RP而改变。ARMD组的染色强度较低，但分布相似。Tau-2 IR微弱，不随年龄变化，但在RP眼和ARMD眼的视网膜色素上皮（RPE）中轻度升高。APP IR在神经节细胞和神经纤维层中最为突出，在老年人神经节细胞和RP和ARMD眼的RPE细胞中出现增加。β -淀粉样蛋白IR仅在老年人眼睛的亚rpe沉积物中局部检测到。结论：本研究中研究的蛋白存在于人视网膜中。tau的染色模式与大脑不同，但它没有显示出与年龄相关的变化。APP在老年眼视网膜神经节细胞和RP和ARMD眼RPE细胞中的免疫反应性增加，以及RPE亚沉积物中β -淀粉样蛋白的斑片状染色，可能表明这些蛋白与RP视网膜老化有关，可能与视网膜变性有关。
PURPOSE Increased immunoreactivity (IR) of beta-amyloid and the amyloid-associated proteins tau and amyloid precursor protein (APP) in the brain have been linked to the pathogenesis of neurodegenerative disorders such as Alzheimer's disease. However, the expression of these proteins has not been investigated in the normal or diseased human retina. METHODS Using immunohistochemical techniques, we examined the distribution and age-related changes of anti-tau-1, anti-tau-2, anti-APP, and anti-beta-amyloid IR in the human retina at various ages (n = 24), in retinitis pigmentosa (RP, n = 6), and in age-related macular degeneration (ARMD, n = 10). RESULTS Tau-1 immunoreactivity was intense in the inner retinal layers and did not change with age or in RP. Eyes with ARMD showed less intense staining but exhibited a similar distribution. Tau-2 IR was faint and did not change with age but was mildly increased in the retinal pigment epithelium (RPE) of eyes with RP and in the retina of eyes with ARMD. APP IR was most prominent in the ganglion cell and nerve fiber layer, and it appeared to increase in ganglion cells of older persons and in RPE cells of eyes with RP and ARMD. Beta-amyloid IR was only detected focally in sub-RPE deposits in eyes from older persons. CONCLUSIONS The proteins investigated in this study are present in the human retina. The staining pattern of tau is different from the brain, but it shows no age-related changes. The increased immunoreactivity of APP in retinal ganglion cells of older eyes and in RPE cells of eyes with RP and ARMD, as well as the patchy staining of beta-amyloid within sub-RPE deposits, might indicate a relationship of these proteins to retinal aging and possibly to retinal degeneration in RP.