Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species
Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species
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盐诱导耐盐和盐敏感杨树叶片中 Na/K 和 ROS 稳态相关基因的表达
DOI：
10.1007/s11103-010-9612-9
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Chen, Shaoliang
中科院分区：
文献类型：
--
作者：
Ding, Mingquan;Hou, Peichen;Chen, Shaoliang
利用白杨阵列，我们探索了耐盐胡杨叶片转录组。和盐敏感的P. popularis 35-44（P. popularis）。我们的目的是阐明两个物种在调节K（+）/Na（+）和活性氧（ROS）稳态方面的基因组差异。与胡杨相比，耐盐胡杨对盐度的反应涉及在短期（ST）暴露于150 mM NaCl（24 h）后诱导相对较大数量的probesets，而在长期（LT）暴露于盐度（200 mM NaCl，28天）后诱导相对较少的probesets。与胡杨相比，对照胡杨叶片中Na（+）/H（+）反向转运（Na（+）/H（+）antiporters，H（+）pumps）和K（+）吸收转运相关基因的转录丰度更高。值得注意的是，这些基因的表达在盐处理期间没有减少（少数例外）。在活性氧稳态方面，胡杨在盐胁迫下表现出多种抗氧化酶的快速上调，表明其对盐胁迫的快速适应性反应。然而，NaCl对杨叶片中转录的影响在长时间的盐暴露后更加明显。低温胁迫下，杨树林中K（+）/Na（+）稳态调节基因表达上调，超氧自由基和H2 O2清除剂的转录水平下降，但少数清除剂的某些亚型表达下降。矿质元素和活性氧分析结果表明，NaCl胁迫下两种杨树叶片Na+和H2 O2含量显著增加，盐敏感白杨叶片Na+和H2 O2含量增加更明显。我们把我们的生理测量的背景下的转录结果推断NaCl诱导的基因表达的改变与K（+）/Na（+）和ROS的稳态的一些影响。
Using the Affymetrix poplar genome array, we explored the leaf transcriptome of salt-tolerant Populus euphratica Oliv. and salt-sensitive P. popularis 35-44 (P. popularis) under control and saline conditions. Our objective was to clarify the genomic differences in regulating K(+)/Na(+) and reactive oxygen species (ROS) homeostasis between the two species. Compared to P. popularis, salt-tolerant P. euphratica responses to salinity involved induction of a relatively larger number of probesets after short-term (ST) exposure to 150 mM NaCl (24 h) and relatively fewer probesets after a long-term (LT) exposure to salinity (200 mM NaCl, 28 days). Compared to P. popularis, leaves of the control P. euphratica plants exhibited a higher transcript abundance of genes related to Na(+)/H(+) antiport (Na(+)/H(+) antiporters, H(+) pumps) and K(+) uptake and transport. Notably, the expression of these genes did not decrease (with a few exceptions) during salt treatment. Regarding ROS homeostasis, P. euphratica exhibited rapid up-regulation of a variety of antioxidant enzymes after exposure to ST salinity, indicating a rapid adaptive response to salt stress. However, the effect of NaCl on transcription in P. popularis leaves was more pronounced after exposure to prolonged salinity. LT-stressed P. popularis up-regulated some genes mediating K(+)/Na(+) homeostasis but decreased transcription of main scavengers of superoxide radicals and H(2)O(2) except for some isoforms of a few scavengers. Mineral and ROS analyses show that NaCl induced a marked increase of leaf Na(+) and H(2)O(2) in LT-stressed plants of the two species and the effects were even more pronounced in the salt-sensitive poplar. We place the transcription results in the context of our physiological measurements to infer some implications of NaCl-induced alterations in gene expression related to K(+)/Na(+) and ROS homeostasis.