TALEN-Mediated Mutagenesis and Genome Editing.
TALEN-Mediated Mutagenesis and Genome Editing.
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Talen介导的诱变和基因组编辑。
DOI：
10.1007/978-1-4939-3771-4_2
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ekker SC
中科院分区：
文献类型：
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作者：
Ma AC;Chen Y;Blackburn PR;Ekker SC
类转录激活因子效应器 (TALE) 是重要的基因组工具，具有可定制的 DNA 结合基序，可进行位点特异性修饰。特别是，TALE 核酸酶或 TALEN 已成功用于斑马鱼模型系统，通过非同源末端连接 (NHEJ) 或在供体模板存在的情况下通过同源定向修复 (HDR) 和同源无关修复修复双链断裂 (DSB) 来引入靶向突变。与其他可定制核酸酶相比，TALEN 在靶向基因组方面具有较高的结合特异性和较少的序列限制，并且具有相当的诱变活性。在这里，我们描述了使用 Mojo Hand 2.0 软件对 TALEN 和 CRISPR/Cas9 定制限制性酶进行斑马鱼基因组编辑的详细计算机设计工具。
Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA binding motifs for locus-specific modifications. In particular, TALE Nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double stranded breaks (DSBs) either through non-homologous end joining (NHEJ), or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.