Molecular characterization and expression patterns of stem-loop binding protein (SLBP) genes in protogynous hermaphroditic grouper, Epinephelus coioides
Molecular characterization and expression patterns of stem-loop binding protein (SLBP) genes in protogynous hermaphroditic grouper, Epinephelus coioides
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斜带石斑鱼先熟雌雄同体茎环结合蛋白（SLBP）基因的分子特征和表达模式
DOI：
10.1016/j.gene.2019.02.092
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Lin Haoran
中科院分区：
文献类型：
--
作者：
Wu Xi;Qu Ling;Li Shuisheng;Guo Yin;He Jianan;Liu Meifeng;Liu Xiaochun;Lin Haoran
茎环结合蛋白（SLBP）结合 mRNA 的茎环结构，这对于组蛋白 mRNA 和翻译过程的稳定性很重要。在本研究中，从原生雌雄同体橙斑石斑鱼（斜带石斑鱼）中克隆了两个slbpcDNA（Ecslbp1和Ecslbp2）。Ecslbp1cDNA包含一个678个碱基对（bp）的开放阅读框（ORF），编码225个氨基酸的预测多肽。Ecslbp2cDNA包含一个1041bp，编码预测的蛋白质346个氨基酸。实时PCR结果显示Ecslbp2mRNA仅在卵巢中检测到。此外，根据原位杂交（ISH）分析，发现Ecslbp2仅限于卵母细胞。Ecslbp2在原虫中几乎检测不到，并且在初级生长阶段卵母细胞的细胞质中显着增加，但在卵黄发生过程中减少。有趣的是，Ecslbp2的表达集中在初级生长早期卵母细胞中的核周斑点，在初级生长晚期卵母细胞中似乎形成了巴尔比亚尼体（Bb）。这些数据表明Ecslbp2可能在卵母细胞发育过程中发挥重要作用，可以作为石斑鱼卵巢发育和性逆转研究的卵母细胞特异性分子标记。
Stem-loop binding protein (SLBP) binds a stem-loop structure of the mRNA, which is important for the stability of histone mRNAs and translation process. In the present study, twoslbpcDNAs (Ecslbp1andEcslbp2) were cloned from a protogynous hermaphroditic orange-spotted grouper, Epinephelus coioides.Ecslbp1cDNA contained a 678 base pair (bp) open reading frame (ORF), encoding a predicted polypeptide of 225 amino acids.Ecslbp2cDNA contained a 1041 bp, encoding a predicted protein of 346 amino acids. The result of real-time PCR revealed thatEcslbp2mRNA was exclusively detected in the ovary. Moreover, it was found to be restricted to oocytes according to in situ hybridization (ISH) analysis.Ecslbp2was found to be hardly detected in gonia and significantly increase in the cytoplasm of primary-growth stage oocytes, but decreased during the process of vitellogenesis. Interestingly,Ecslbp2expression centralized as a perinuclear speckle in early-primary-growth stage oocytes, which appeared to form into the Balbiani body (Bb) in late-primary-growth stage oocytes. These data indicated thatEcslbp2might play an important role in the process of oocyte development, and could serve as an oocyte-specific molecular marker for the study of ovary development and sex reversal in groupers.