Agricultural activities affect the pattern of the resistome within the phyllosphere microbiome in peri-urban environments.
Agricultural activities affect the pattern of the resistome within the phyllosphere microbiome in peri-urban environments.
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DOI：
10.1016/j.jhazmat.2019.121068
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发表时间：
2020-01
影响因子：
13.6
通讯作者：
Qian Xiang;Dong Zhu;M. Giles;R. Neilson;Xiao-Ru Yang;M. Qiao;Qing‐Lin Chen
中科院分区：
文献类型：
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作者：
Qian Xiang;Dong Zhu;M. Giles;R. Neilson;Xiao-Ru Yang;M. Qiao;Qing‐Lin Chen
植物微生物组是人类暴露于环境抗生素耐药性的关键途径。然而，很少有信息是关于植物相关的抗性基因组在人类相关的环境中，在更大的规模。在这里，通过高通量定量PCR芯片阵列和扩增子测序，我们的特点是抗生素耐药基因（ARG）和细菌群落的植物和土壤微生物组从人类高度干扰城郊农田和干扰较少的森林在流域尺度。在叶球中共检测到71种ARG，几乎涵盖了人类和动物常用的所有主要公认抗生素类别。农田和森林环境中植物抗性组的总体格局存在显著差异（PERMANOVA，P< 0.01），表明农业活动可能是形成植物抗性组的重要驱动因素。二分网络分析表明，在植物微生物组中检测到的所有ARGs也存在于土壤微生物组中。总之，我们的研究结果提供了一个更好的了解植物抗性组，并建议土地利用是一个关键的贡献者的组成ARG配置文件中的植物叶际，土壤抗性组可能代表一个重要的水库植物相关的ARGs。
The plant microbiome represents a crucial pathway for human exposure to environmental antibiotic resistance. However, little information is available regarding the plant associated resistome in human-related environments at a larger scale. Here, by high-throughput quantitative-PCR chip-based array and amplicon sequencing, we characterized antibiotic resistance genes (ARGs) and bacterial communities in plant and soil microbiomes from human highly disturbed peri-urban farmland and less disturbed forest at a watershed scale. A total of 71 ARGs were detected in the phyllosphere, which covered almost all the major recognized classes of antibiotics that are administered commonly to humans and animals. The overall pattern of the plant associated resistome in intensive anthropogenic influenced farmland was significantly different from that of forest environments (PERMANOVA,P< 0.01), indicating that agricultural activities might be important drivers in shaping the plant resistome. A bipartite network analysis suggested that all ARGs detected in the plant microbiome were also present in the soil microbiome. Together, our findings provide a better understanding of the plant resistome and suggest that land use is a key contributor to the composition of ARG profiles in the plant phyllosphere, and that the soil resistome may represent a critical reservoir of plant associated ARGs.