Fluorescence-enhanced optical imaging in large tissue volumes using a gain-modulated ICCD camera
Fluorescence-enhanced optical imaging in large tissue volumes using a gain-modulated ICCD camera
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DOI：
10.1088/0031-9155/48/12/303
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发表时间：
2003-06-21
影响因子：
3.5
通讯作者：
Sevick-Muraca, EM
中科院分区：
文献类型：
--
作者：
Godavarty, A;Eppstein, MJ;Sevick-Muraca, EM
一种新型图像增强电荷耦合器件 (ICCD) 成像系统已被开发出来，可使用近红外造影剂在频域中执行 3D 荧光断层成像。该成像仪的独特之处在于：(i) 采用大型组织模拟体模，其形状和尺寸类似于女性乳房和扩展胸壁区域的一部分；(ii) 通过使用增益调制 ICCD 相机实现频域中的快速数据采集。将扩散模型预测与在荧光剂完美和不完美摄取到目标的两种不同实验条件下使用两种不同参考方案的实验测量进行比较。根据这些实验测量，使用近似扩展卡尔曼滤波器算法的计算高效变体重建了荧光吸收的三维图像。目前的工作首次通过使用增益调制 ICCD 相机对临床相关乳房形状组织模型上的时间依赖性光传播进行实验测量来实现 3D 荧光增强光学断层扫描重建。
A novel image-intensified charge-coupled device (ICCD) imaging system has been developed to perform 3D fluorescence tomographic imaging in the frequency-domain using near-infrared contrast agents. The imager is unique since it (i) employs a large tissue-mimicking phantom, which is shaped and sized to resemble a female breast and part of the extended chest-wall region, and (ii) enables rapid data acquisition in the frequency-domain by using a gain-modulated ICCD camera. Diffusion model predictions are compared to experimental measurements using two different referencing schemes under two different experimental conditions of perfect and imperfect uptake of fluorescent agent into a target. From these experimental measurements, three-dimensional images of fluorescent absorption were reconstructed using a computationally efficient variant of the approximate extended Kalman filter algorithm. The current work represents the first time that 3D fluorescence-enhanced optical tomographic reconstructions have been achieved from experimental measurements of the time-dependent light propagation on a clinically relevant breast-shaped tissue phantom using a gain-modulated ICCD camera.