Normal and leukemic human stem cells assayed in SCID mice.
Normal and leukemic human stem cells assayed in SCID mice.
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DOI：
10.1006/smim.1996.0025
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发表时间：
1996-08-01
影响因子：
7.8
通讯作者：
Dick, J E
中科院分区：
文献类型：
--
作者：
Dick, J E
了解调节正常干细胞和引发增殖性疾病（如白血病）的干细胞发育程序的过程仍然是生物学的主要挑战之一。直到最近，由于缺乏正常和白血病干细胞的体内测定，解决人类造血系统中这些主要问题的进展一直受到阻碍。近年来，将正常和白血病造血细胞移植到免疫缺陷小鼠体内的方法的发展为干细胞的鉴定、表征和纯化提供了重要途径。这篇评论将集中在正常和白血病的人类干细胞的检测方法的发展和新的见解，这些检测方法开始提供组织的人类干细胞层次结构和机制的白血病。
Understanding the processes that regulate the developmental program of normal stem cells and those that initiate proliferative diseases such as leukemia remains one of the major challenges in biology. Progress to address these major questions in the human hematopoietic system have been hampered, until recently, by the lack of in-vivo assays for normal and leukemic stem cells. The recent development of methods to transplant normal and leukemic human hematopoietic cells into immune-deficient mice provides an important approach to identify, characterize and purify stem cells. This review will focus on the development of assays for normal and leukemic human stem cells and on the new insights these assays are beginning to provide on the organization of the human stem cell hierarchy and mechanisms of leukemogenesis.