Transient increase of microglial C1q expression in the circumventricular organs of adult mouse during LPS-induced inflammation.
Transient increase of microglial C1q expression in the circumventricular organs of adult mouse during LPS-induced inflammation.
复制标题
LPS 诱导的炎症期间成年小鼠心室周围器官中小胶质细胞 C1q 表达的短暂增加。
DOI：
10.1002/cbf.3477
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Miyata S.
中科院分区：
文献类型：
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作者：
Kawai S;Kurganov E;Miyata S.
脑室周围器官（CVO）是缺乏血脑屏障并允许血液衍生分子自由进入的大脑区域，为在大脑中启动快速和早期神经炎症反应提供了专门的利基。补体成分1 q（C1 q）被证明是补体途径的第一识别成分，并且在病理条件下在大脑中具有至关重要的功能。在本研究中，我们发现，在单次给予1 mg/kg脂多糖（LPS）后1天，CX 3CR 1阳性小胶质细胞中的C1 q表达在成年小鼠的CVOs及其邻近脑区中增加，而在LPS刺激后3天恢复到对照水平。C1 q表达也被发现定位于不同脑区的突触蛋白阳性突触前轴突终末。因此，本研究证实了C1 q在CVOs及其邻近脑区的瞬时上调表达，表明C1 q的瞬时上调可能与脑炎症早期的生理反应有关。研究意义脑室周围器官（CVOs）是缺乏血脑屏障（BBB）的脑内特化区域，启动脑内的神经炎症反应。目前的研究表明，补体蛋白C1 q的表达高度增加，在小胶质细胞的CVOs及其邻近的脑区。此外，C1 q的表达被观察到专门定位在突触前轴突终末的CVOs和他们的邻近脑区。因此，本研究表明，C1 q可能与脑炎症早期的生理反应有关。
The circumventricular organs (CVOs) are the brain regions that lack the blood‐brain barrier and allow free entry of blood‐derived molecules, offering specialized niche to initiate rapid and early neuroinflammatory responses in the brain. Complement component 1q (C1q) is shown to be the first recognition component of the complement pathway and has a crucial function in the brain under pathological conditions. In the present study, we found that C1q expression in CX3CR1‐positive microglia was increased in the CVOs and their neighbouring brain regions of adult mice at 1 day after a single administration of 1 mg/kg lipopolysaccharide (LPS), whereas it returned to control levels at 3 days after LPS stimulation. C1q expression was also seen to localize at synapsin‐positive presynaptic axonal terminals in various brain regions. Thus, the present study demonstrates a transient upregulation of microglial C1q expression in the CVOs and their adjacent brain regions, indicating that a transient upregulation of C1q is possibly concerned with physiological responses at early phase of brain inflammation.Significance of the StudyThe circumventricular organs (CVOs) are specialized brain regions that lack the blood‐brain barrier (BBB) and initiate neuroinflammatory responses in the brains. The present study showed that the expression of complement protein C1q was highly increased in microglia of the CVOs and their adjacent brain regions. Moreover, C1q expression was observed to localize specifically at presynaptic axonal terminals in the CVOs and their neighbouring brain regions. Thus, the present study indicates that C1q is possibly correlated with physiological responses at early phase of brain inflammation.