Dopamine-beta-hydroxylase inhibitors modulate the concentration of functional estrogen receptors in female rat hypothalamus and pituitary gland.
Dopamine-beta-hydroxylase inhibitors modulate the concentration of functional estrogen receptors in female rat hypothalamus and pituitary gland.
复制标题
多巴胺-β-羟化酶抑制剂调节雌性大鼠下丘脑和垂体中功能性雌激素受体的浓度。
DOI：
10.1159/000124522
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发表时间：
1986
影响因子：
4.1
通讯作者：
Swearengen,ES
中科院分区：
文献类型：
--
作者：
Blaustein,JD;Brown,TJ;Swearengen,ES
为了进一步研究儿茶酚胺对去卵巢大鼠脑和垂体前叶类固醇激素受体的调节作用，我们研究了两种多巴胺-β-羟化酶抑制剂对雌激素受体系统的影响。在缺乏雌二醇的情况下，FLA 63和二乙基二硫代氨基甲酸酯（DDC）两种抑制剂均对下丘脑中基底细胞浆雌激素受体的浓度产生双相影响，而不影响细胞核雌激素受体的浓度；处理后细胞质雌激素受体浓度先升高后降低。在一项对多巴胺-β-羟化酶抑制降低胞质雌激素受体浓度的神经解剖区域的研究中，发现药物治疗后，下丘脑中基底区和视前隔区胞质雌激素受体浓度下降，但杏仁核中没有。这表明多巴胺-β-羟化酶抑制剂引起的下丘脑细胞质雌激素受体浓度的降低并不是对所有细胞质雌激素受体的非特异性作用。最后，在用DDC或FLA 63预处理过的动物中注射雌二醇-17β，在药物首先引起细胞质雌激素受体浓度下降的组织中，细胞核雌激素受体的积累比用载体注射的对照组少。因此，在某些条件下，多巴胺-β-羟化酶抑制剂预处理降低了功能性细胞质雌激素受体的浓度，导致雌二醇注射后核雌激素受体积累减少。这些结果支持了一种假设，即儿茶酚胺在调节某些下丘脑神经元对雌二醇的敏感性中起作用，在某些情况下，在垂体前叶中起作用。
In order to study further the regulation of steroid hormone receptors in the brain and anterior pituitary gland of ovariectomized rats by catecholamines, the influence of two dopamine-β-hydroxylase inhibitors on the estrogen receptor system was studied. The two inhibitors, FLA 63 and diethyldithiocarbamate (DDC) both caused a biphasic effect on the concentration of cytosol estrogen receptors in the mediobasal hypothalamus without influencing the concentration of cell nuclear estrogen receptors in the absence of estradiol; the concentration of cytosol estrogen receptors first increased and then decreased after treatment. In a study of the neuroanatomical areas in which inhibition of dopamine-β-hydroxylase decreases the concentration of cytosol estrogen receptors, it was found that the concentration of cytosol estrogen receptors decreased after drug treatment in the mediobasal hypothalamus and preoptic area-septum, but not in the amygdala. This suggests that the decrease in concentration of hypothalamic cytosol estrogen receptors caused by dopamine-β-hydroxylase inhibitors is not a nonspecific effect on all cytosol estrogen receptors. Finally, an injection of estradiol-17β in animals that had been pretreated with either DDC or FLA 63 resulted in less accumulation of cell nuclear estrogen receptors in those tissues in which the drug first caused a decrease in the concentration of cytosol estrogen receptors than in vehicle-injected controls. Therefore, under some conditions, pretreatment with a dopamine-β-hydroxylase inhibitor decreased the concentration of functional cytosol estrogen receptors resulting in decreased nuclear estrogen receptor accumulation in response to an estradiol injection. These results support the hypothesis that the catecholamines have a role in modulating the sensitivity to estradiol in some hypothalamic neurons and, in some circumstances, in the anterior pituitary gland.