Superoxide dismutase in human sperm suspensions: Relationship with cellular composition, oxidative stress, and sperm function
Superoxide dismutase in human sperm suspensions: Relationship with cellular composition, oxidative stress, and sperm function
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DOI：
10.1016/0891-5849(96)00119-0
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发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Irvine, DS
中科院分区：
文献类型：
--
作者：
Aitken, RJ;Buckingham, DW;Irvine, DS
已经开发出用于监测人类精子制剂中超氧化物歧化酶（SOD）活性的灵敏技术。与通常赋予 SOD 的保护作用相反，从 Percoll 梯度的低密度区域回收的有缺陷精子群体的 SOD 含量比在高密度 Percoll 中沉淀的功能健全的制剂多三倍。 SOD活性与人类精子的运动特性及其卵母细胞融合能力呈负相关，与过氧化损伤的诱导呈正相关。 SOD 活性还与细胞质空间的其他标志物、肌酸激酶 (CK) 和葡萄糖-6-磷酸脱氢酶 (G-6-PDH) 高度相关。我们的结论是，虽然 SOD 可能在维持 O-2(.-) 和 H2O2 之间的平衡方面发挥生理作用，但高水平的这种酶与精子功能受损有关，因为 (a) 人类精子对 H2O2 的细胞毒性作用高度敏感，(b) O-2(.-) 是正常精子功能的重要介质，(c) 高 SOD 活性反映了与生殖细胞脱落相关的精子发生错误以及精子细胞保留多余的残留细胞质。精子。
Sensitive techniques have been developed for monitoring superoxide dismutase (SOD) activities in human sperm preparations. In contradiction to the protective role normally assigned to SOD, populations of defective spermatozoa recovered from the low density region of Percoll gradients were found to have three times more SOD than functionally competent preparations pelleting in high density Percoll. SOD activity was negatively correlated with the movement characteristics of human spermatozoa and their capacity for oocyte fusion, and positively associated with the induction of peroxidative damage. SOD activity was also highly correlated with other markers of the cytoplasmic space, creatine kinase (CK), and glucose-6-phosphate dehydrogenase (G-6-PDH). We conclude that while SOD may play a physiological role in maintaining a balance between O-2(.-) and H2O2, high levels of this enzyme are associated with impaired sperm function because (a) the human spermatozoon is highly susceptible to the cytotoxic effects of H2O2, (b) O-2(.-) is an important mediator of normal sperm function, and (c) high SOD activities reflect errors in spermatogenesis associated with germ cell exfoliation and the retention of excess residual cytoplasm by the spermatozoa.