An expanded family of fungalysin extracellular metallopeptidases of Coprinopsis cinerea
An expanded family of fungalysin extracellular metallopeptidases of Coprinopsis cinerea
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DOI：
10.1016/j.mycres.2007.11.013
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发表时间：
2008-03-01
影响因子：
--
通讯作者：
Gathman, Allen C.
中科院分区：
文献类型：
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作者：
Lilly, Walt W.;Stajich, Jason E.;Gathman, Allen C.
蛋白水解酶，特别是真菌来源的分泌型蛋白酶，是最重要的工业酶之一，但这些蛋白的生化性质和底物特异性一直难以表征。基因组测序为鉴定潜在的新型蛋白酶提供了有力的工具。模式担子菌灰盖鬼伞的基因组被发现具有异常高数量的金属蛋白酶，其与称为真菌溶素的M36肽酶家族紧密匹配。8个预测C.与来自其它真菌的真菌溶素相比，灰霉菌溶素分为两类。一个成员，CcMEP 1，是最相似的单一代表性的真菌溶素从担子菌黄孢原毛革菌，新型隐球菌，和玉米黑粉菌，和真菌溶素型蛋白从烟曲霉。剩下的7个C。cinereus预测的真菌溶素形成与双色蜡蘑的三种预测的M36肽酶相似的组。所有八个C。cinereus酶含有标志性M36 Pfam结构域和FTP前肽结构域。所有包含大的前肽具有相当大的序列保守性附近的一个建议的切割位点。预测的成熟酶的大小范围为37-46 kDa，等电点为弱酸性至中性。这些基因与端粒和/或转座因子的接近可能有助于该基因家族在C.灰色的(C)2007年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Proteolytic enzymes, particularly secreted proteases of fungal origin, are among the most important of industrial enzymes, yet the biochemical properties and substrate specificities of these proteins have been difficult to characterize. Genomic sequencing offers a powerful tool to identify potentially novel proteases. The genome of the model basidiomycete Coprinopsis cinereus was found to have an unusually high number of metalloproteases that closely match the M36 peptidase family known as fungalysins. The eight predicted C. cinereus fungalysins divide into two groups upon comparison with fungalysins from other fungi. One member, CcMEP1, is most similar to the single representative fungalysins from the basidiomycetes Phanerochaete chrysosporium, Cryptococcus neoformans, and Ustilago maydis, and to the fungalysin type-protein from Aspergillus fumigatus. The remaining seven C. cinereus predicted fungalysins form a group with similarity to three predicted M36 peptidases of Laccaria bicolor. All eight of the C. cinereus enzymes contain both the signature M36 Pfam domain and the FTP propeptide domain. All contain large propeptides with considerable sequence conservation near a proposed cleavage site. The predicted mature enzymes range in size from 37-46 kDa and have isoelectric points that are mildly acidic to neutral. The proximity of these genes to telomeres and/or to transposable elements may have contributed to the expansion of this gene family in C. cinereus. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.