Peptide functionalized gold nanoparticles: the influence of pH on binding efficiency
Peptide functionalized gold nanoparticles: the influence of pH on binding efficiency
复制标题
DOI：
10.1088/1361-6528/aa77ac
复制
发表时间：
2017-07-21
期刊：
影响因子：
3.5
通讯作者：
Dixon, Dorian
中科院分区：
文献类型：
--
作者：
Harrison, Emma;Hamilton, Jeremy W. J.;Dixon, Dorian
本文报道了用聚乙二醇（PEG）和三种肽中的一种封端的混合单层金纳米颗粒（AuNPs）的合成。受体介导的内吞作用肽、内体逃逸途径（H5 WYG）肽或FITC标记的Nrp-1靶向RGD肽（CRGDK）。所有三种肽都具有含巯基的半胱氨酸残基，其可用于将肽结合至AuNP。为了研究pH对肽附着的影响，将PEG化的AuNP离心，除去上清液，然后将纳米颗粒重悬于高于、低于和处于共官能化之前肽的相应等电点的pH缓冲溶液范围中。利用动态光散射、紫外-可见光谱（UV/维斯）、FTIR和光致发光光谱研究了肽的附着。UV/维斯分析结合蛋白质测定结果和FITC标记的RGD肽的光致发光得出结论，接近8的pH优化了半胱氨酸结合和稳定性，而与所使用的肽无关。
We report herein on the synthesis of mixed monolayer gold nanoparticles (AuNPs) capped with both polyethylene glycol (PEG) and one of three peptides. Either a receptor-mediated endocytosis peptide, an endosomal escape pathway (H5WYG) peptide or the Nrp-1 targeting RGD peptide (CRGDK) labeled with FITC. All three peptides have a thiol containing cysteine residue which can be used to bind the peptides to the AuNPs. In order to investigate the influence of pH on peptide attachment, PEGylated AuNPs were centrifuged, the supernatant removed, and the nanoparticles were then re-suspended in a range of pH buffer solutions above, below and at the respective isoelectric points of the peptides before co-functionalization. Peptide attachment was investigated using dynamic light scattering, Ultra-violet visible spectroscopy (UV/Vis), FTIR and photo luminescence spectroscopy. UV/Vis analysis coupled with protein assay results and photoluminescence of the FITC tagged RGD peptide concluded that a pH of similar to 8 optimized the cysteine binding and stability, irrespective of the peptide used.