Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels
Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels
复制标题
通过溶胀微凝胶的超分辨率显微镜定量功能交联剂反应动力学
DOI：
10.1039/c9sm01618j
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发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
Ullal, Chaitanya K.
中科院分区：
文献类型：
--
作者：
Karanastasis, Apostolos A.;Kenath, Gopal S.;Sundararaman, Ravishankar;Ullal, Chaitanya K.
超分辨率显微镜（SRM）带来了光学显微镜的优势，纳米结构的软物质的成像，并在胶体微凝胶，有望量化的交联密度在前所未有的长度尺度的变化。然而，所有交联的分布与染料标记的交联的分布不一致，并且由于染料分子计数过多/不足，无法保证SRM中的密度定量。在这里，我们表明，SRM图像的微凝胶编码的反应速率常数的功能交联剂，这是关键，相关的这些分布。结合微凝胶颗粒半径的演变，功能交联剂分布预测消费与时间的高保真度。使用贝叶斯回归的方法，我们提取的反应速率常数为同质和交叉传播的功能交联剂，这应该是广泛有用的预测凝胶的交联密度的空间变化。
Super resolution microscopy (SRM) brings the advantages of optical microscopy to the imaging of nanostructured soft matter, and in colloidal microgels, promises to quantify variations of crosslink densities at unprecedented length scales. However, the distribution of all crosslinks does not coincide with that of dye-tagged crosslinks, and density quantification in SRM is not guaranteed due to over/under-counting dye molecules. Here we demonstrate that SRM images of microgels encode reaction rate constants of functional cross linkers, which hold the key to correlating these distributions. Combined with evolution of microgel particle radii, the functional cross linker distributions predict consumption versus time with high fidelity. Using a Bayesian regression approach, we extract reaction rate constants for homo and cross propagation of the functional crosslinker, which should be widely useful for predicting spatial variations in crosslink density of gels.