A novel surface plasmon resonance imaging interferometry for protein array detection
A novel surface plasmon resonance imaging interferometry for protein array detection
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DOI：
10.1016/j.snb.2007.07.106
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发表时间：
2008-03
影响因子：
8.4
通讯作者：
Xinglong Yu;Xiang Ding;Fangfang Liu;Yan Deng
中科院分区：
文献类型：
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作者：
Xinglong Yu;Xiang Ding;Fangfang Liu;Yan Deng
提出了一种基于时域相位调制干涉的表面等离子体共振（SPR）系统。该系统的特点是，当光从传感表面反射出来，经过调制控制器控制的电光晶体时，反射光的p偏振分量和s偏振分量之间的相位差被调制形成干涉。同步相位调制和信号采集，利用Stoilov算法对数据进行处理，得到各传感单元中折射率变化引起的反射光的相位变化。它是一种完整的共光路检测方法，可以抑制光噪声，提高检测灵敏度。利用该检测方法构建的实验装置，进行了NaCl溶液检测实验和生物分子相互作用实验。实验结果和分析表明，该方法可实现10−6折射率单位（RIU）以上的分辨率，且单个CCD像元可感知一个检测单元。为了降低噪声，可以使用2×2或3×3像素来检测一个检测单元，并且也可以轻松实现高吞吐量。因此，时域相位调制干涉测量技术在诊断、药物发现和开发等方面具有广阔的应用前景。
A novel surface plasmon resonance (SPR) system based on time domain phase modulation interference is presented. The characteristic of the system is that when light is reflected from the sensing surface and passes through the electro-optical crystal controlled by modulation controller, the phase difference between p- and s-polarized components of the reflected light is modulated to form interference. Synchronizing phase modulation and signal acquisition and using Stoilov algorithm to process data, phase change of the reflected light caused by refractive index variation in each sensing unit can be obtained. It is a complete common-light path detection method that can restrain light noise and improve detection sensitivity. Utilizing the experimental device constructed in this detection method, detection of NaCl solution experiments and biomolecular interaction experiments was performed. Experimental results and analysis show that a resolution higher than 10−6refractive index unit (RIU) is expected to be achieved, and one single CCD pixel enables sensing a detection unit. To reduce noises, 2×2 or 3×3 pixels can be used to detect one detection unit and high throughput can also be achieved easily. Thus, time domain phase modulation interferometry is potential to be applied in diagnosis, drug discovery and development.