Positive no-touch surfaces and undetectable SARS-CoV-2 aerosols in long-term care facilities: An attempt to understand the contributing factors and the importance of timing in air sampling campaigns.
Positive no-touch surfaces and undetectable SARS-CoV-2 aerosols in long-term care facilities: An attempt to understand the contributing factors and the importance of timing in air sampling campaigns.
复制标题
DOI：
10.1016/j.ajic.2021.02.004
复制
发表时间：
2021-06
影响因子：
4.9
通讯作者：
Duchaine C
中科院分区：
文献类型：
--
作者：
Dumont-Leblond N;Veillette M;Bhérer L;Boissoneault K;Mubareka S;Yip L;Dubuis ME;Longtin Y;Jouvet P;McGeer A;Duchaine C
长期护理设施（LTCF）是特别有利于冠状病毒疾病（SARS-CoV-2）大流行爆发的环境，因为它们欢迎高危人群，而且与居民的距离很近。然而，该疾病在这些场所的传播动态仍不清楚。采集7个长期护理中心31个房间的空气和非接触表面，采用实时逆转录聚合酶链反应（RT-qPCR）定量检测SARS-CoV-2。空气样本呈阴性，但从62个表面样本中的20个样本中回收了病毒基因组，浓度范围为13至36,612个基因组/表面。表面样品（n = 7）的病毒分离（培养）结果为阴性。在非接触表面上存在病毒RNA是病毒通过空气传播的证据，但空气样本中缺乏空气传播的病毒颗粒表明，在空气采样期间，它们没有以重要的方式雾化。空气样本采集时间为居民症状出现后8 ~ 30天，说明病毒在感染过程中较早被雾化。需要进一步的研究来评估这些机构中病毒活力保存以及直接接触和气溶胶在SARS-CoV-2传播中的潜在作用。
Long-term care facilities (LTCF) are environments particularly favorable to coronavirus disease (SARS-CoV-2) pandemic outbreaks, due to the at-risk population they welcome and the close proximity of residents. Yet, the transmission dynamics of the disease in these establishments remain unclear. Air and no-touch surfaces of 31 rooms from 7 LTCFs were sampled and SARS-CoV-2 was quantified by real-time reverse transcription polymerase chain reaction (RT-qPCR). Air samples were negative but viral genomes were recovered from 20 of 62 surface samples at concentrations ranging from 13 to 36,612 genomes/surface. Virus isolation (culture) from surface samples (n = 7) was negative. The presence of viral RNA on no-touch surfaces is evidence of viral dissemination through air, but the lack of airborne viral particles in air samples suggests that they were not aerosolized in a significant manner during air sampling sessions. The air samples were collected 8 to 30 days after the residents’ symptom onset, which could indicate that viruses are aerosolized early in the infection process. Additional research is needed to evaluate viral viability conservation and the potential role of direct contact and aerosols in SARS-CoV-2 transmission in these institutions.
登录
查看更多内容
影响因子：
64.5
作者：
Hou, Yixuan J.;Okuda, Kenichi;Baric, Ralph S.
通讯作者：
Baric, Ralph S.
DOI：
10.1073/pnas.2015482118
发表时间：
2021-02-23
影响因子：
11.1
作者：
Azimi P;Keshavarz Z;Cedeno Laurent JG;Stephens B;Allen JG
通讯作者：
Allen JG
影响因子：
11.1
作者：
Lu, Jing;Peng, Jinju;Ke, Changwen
通讯作者：
Ke, Changwen
影响因子：
9.8
作者：
Ding, Zhen;Qian, Hua;Li, Yuguo
通讯作者：
Li, Yuguo
影响因子：
11.8
作者：
Bullard, Jared;Dust, Kerry;Poliquin, Guillaume
通讯作者：
Poliquin, Guillaume