Ultra long-term cellular tracing by a fluorescent AIE bioconjugate with good water solubility over a wide pH range
Ultra long-term cellular tracing by a fluorescent AIE bioconjugate with good water solubility over a wide pH range
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通过荧光 AIE 生物共轭物进行超长期细胞示踪，在较宽的 pH 范围内具有良好的水溶性
DOI：
10.1039/c7tb00861a
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发表时间：
2017
影响因子：
7
通讯作者：
Tang Ben Zhong
中科院分区：
文献类型：
--
作者：
Wang Zhengke;Yang Ling;Liu Yalan;Huang Xiaofei;Qiao Fenghui;Qin Wei;Hu Qiaoling;Tang Ben Zhong
强荧光和水溶性生物探针在研究重要的生物事件和过程方面有很大的需求。传统有机染料因聚集而产生的猝灭效应，以及无机量子点的高毒性，一直是困扰这一领域科学家的棘手问题。在本工作中，我们成功地将大量的四苯基乙烯(TPE)单元连接到N-琥珀酰壳聚糖(NSCS)大分子链上，制备了一种新型的TPE-NSCS荧光生物结合物，由于其聚集诱导发射效应，在固态下具有强烈的发光特性。TPE-NSCS可在较宽的pH值范围内溶解于水中。令我们惊喜的是，染色的细胞在多达30代的情况下仍然显示出明亮的荧光发射。TPE-NSCS在宽pH范围内的水溶性、在细胞中的超长时间滞留以及强烈的荧光信号表明，TPE-NSCS在各种生物医学应用中具有广阔的应用前景。
Strongly fluorescent and water soluble bioprobes are in great demand for studying important biological events and processes. The quenching effect of conventional organic dyes caused by aggregation, and the high toxicity of inorganic quantum dots are thorny issues that have constantly obsessed scientists in this area of research. In this work, a large number of tetraphenylethene (TPE) units were successfully attached to N-succinyl-chitosan (NSCS) macromolecular chains to fabricate a novel TPE-NSCS fluorescent bioconjugate, which was strongly emissive in the solid state due to its aggregation-induced emission effect. TPE-NSCS could be solubilized in water over a wide range of pH values. We were pleasantly surprised to see that stained cells still showed a bright fluorescence emission for as many as 30 passages. The water solubility over a wide pH range, ultra long-term retention in cells, and strong fluorescence signals indicate that TPE-NSCS is a promising candidate for various biomedical applications.