ATR-FTIR, FT-NIR and near-FT-Raman spectroscopic studies of molecular composition in human skin in vivo and pig ear skin in vitro
ATR-FTIR, FT-NIR and near-FT-Raman spectroscopic studies of molecular composition in human skin in vivo and pig ear skin in vitro
复制标题
DOI：
10.1155/2008/969217
复制
发表时间：
2008-01-01
影响因子：
--
通讯作者：
Nielsen, Ole F.
中科院分区：
文献类型：
--
作者：
Greve, Tanja M.;Andersen, Kristine B.;Nielsen, Ole F.
采用ATR-FTIR、FT-NIR和near-FT-拉曼光谱对人在体皮肤和猪耳离体皮肤的分子组成进行了表征。由于不同的测量深度，光谱技术揭示了皮肤不同层的特征。用ATR-FTIR技术进行胶带剥离。在所有测量的皮肤深度，关于脂质含量和构象，蛋白质二级结构或水含量的光谱差异被发现与性别和物种（即人与猪耳）。对皮肤FT-NIR和ATR-FTIR光谱中未归属的脂类和蛋白质峰进行了新的归属。PCA和PLS模型被用来研究所记录的光谱分组。对于男性和女性受试者的分类，PLS判别分析提供了基于ATR-FTIR光谱的64-93%的分类准确度和基于拉曼光谱的83-89%的分类准确度。对于人体皮肤在体内和猪耳皮肤在体外的分类，PLS判别分析提供了75-100%的分类精度的基础上的拉曼光谱和100%的ATR-FTIR光谱。
ATR-FTIR, FT-NIR and near-FT-Raman spectroscopy were used to characterize the molecular composition of human skin in vivo and pig ear skin in vitro. Due to different measurement depths the spectroscopic techniques reveal the characteristics of different layers of the skin. Tape stripping was used with the ATR-FTIR technique. Spectral differences concerning lipid content and conformation, protein secondary structure or content of water were found with respect to both gender and species (i.e. human versus pig ear) at all measured skin depths. New assignments of so far unassigned lipid and protein peaks in the FT-NIR and ATR-FTIR spectra of skin were made. PCA and PLS models were used to investigate the division of the recorded spectra into groups. With respect to classification of male and female subjects, the PLS discriminant analysis provided a classification accuracy of 64-93% based on the ATR-FTIR spectra and 83-89% based on the Raman spectra. With respect to classification of human skin in vivo and pig ear skin in vitro, the PLS discriminant analysis provided a classification accuracy of 75-100% based on the Raman spectra and 100% based on the ATR-FTIR spectra.