Development of a reliable method to determine monoterpene emission rate of plants grown in an open-top chamber
Development of a reliable method to determine monoterpene emission rate of plants grown in an open-top chamber
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DOI：
10.2480/agrmet.d-15-00002
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发表时间：
2015-12
影响因子：
1.3
通讯作者：
Tomoki Mochizuki;Tasuku Saito;Gou Hirai;M. Miwa;Tetsushi Yonekura
中科院分区：
文献类型：
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作者：
Tomoki Mochizuki;Tasuku Saito;Gou Hirai;M. Miwa;Tetsushi Yonekura
为了测量在广泛使用的开顶室（OTCs）中生长的植物的单萜烯排放，我们开发了一种不涉及刺激叶子和树枝的气体采样方法。在60 × 60 × 120 cm的OTC环境中种植了4个杉木（Cryptomeria japonica）无性系。为了对单萜烯进行取样，采用现有的OTC作为动态包封体系。OTC的开放侧（顶部）使用透明丙烯酸板覆盖，中间有一个孔（直径10厘米）。净化后的空气通过50个孔（直径3mm）供应，孔是在环形聚四氟乙烯管中水平固定在OTC底部附近。下午（n = 8 ~ 10）测得的单萜烯排放率与温度呈良好的相关关系（r 2 = 0.94 ~ 1.00），利用这一关系和记录的温度可以估算出实验某一时间段内的总单萜烯排放率。研究结果表明，利用现有的OTC气体取样系统是一种可靠的方法，可以在不刺激叶子和树枝的情况下收集植物释放的单萜烯。它可以用来比较植物在环境胁迫下的单萜烯释放势。
To measure monoterpene emissions from plants grown in widely used open-top chambers ( OTCs ) , we developed a gas sampling method that does not involve the stimulation of leaves and branches. Four clones of Japanese cedar ( Cryptomeria japonica ) were grown in the OTC ( 60 × 60 × 120 cm ) . To sample the monoterpenes, the existing OTC was used for a dynamic enclosure system. The open side ( top ) of the OTC was covered using a transparent acrylic plate with a hole ( 10 cm in diameter ) in the center. Purified air was supplied through 50 holes ( 3 mm in diameter ) made in a ring-shaped Teflon tube horizontally fixed near the bottom of the OTC. Monoterpene emission rates measured during the afternoon ( n = 8–10 ) were well correlated with temperature ( r 2 = 0.94 – 1.00 ) , and the total monoterpene emission rate during a certain period of the experiment can be estimated using this relationship and recorded temperature. Our results suggest that the developed gas sampling system using the existing OTC is a reliable method to collect monoterpenes emitted from plants without stimulating leaves and branches. It can be used to compare the monoterpene emission poten-tial of plants affected by environmental stresses applied for OTC experiments.