Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics
Volumetric optical coherence microscopy with a high space-bandwidth-time product enabled by hybrid adaptive optics
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DOI：
10.1364/boe.9.003137
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发表时间：
2018-07-01
影响因子：
3.4
通讯作者：
Adie, Steven G.
中科院分区：
文献类型：
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作者：
Liu, Siyang;Mulligan, Jeffrey A.;Adie, Steven G.
光学相干显微镜（OCM）是一种有前途的高分辨率成像方式，但以细胞分辨率捕获有关动态生物过程的大规模体积信息的能力有限。为了提高 OCM 的吞吐量，我们实施了一种混合自适应光学 (hyAO) 方法，该方法将计算自适应光学与通过硬件自适应光学生成的有意像差的成像光束相结合。使用 hyAO，我们展示了像散光束与高斯光束相比的深度均衡照明和收集能力，用于细胞分辨率成像。凭借这一优势，与采用跨深度聚焦扫描的高斯光束采集相比，我们实现了具有更高空间带宽时间乘积的体积 OCM。 HyAO 还用于在 1mm x 1mm x 1mm 视场上执行细胞动力学体积延时 OCM 成像，具有 2 μm 各向同性空间分辨率和 3 分钟时间分辨率。由于 hyAO 与谱域和扫频源光束扫描 OCM 系统兼容，因此通过使用超高速扫频源可以进一步显着提高绝对体积吞吐量。 (C) 2018 年美国光学协会根据 OSA 开放获取出版协议的条款。
Optical coherence microscopy (OCM) is a promising modality for high resolution imaging, but has limited ability to capture large-scale volumetric information about dynamic biological processes with cellular resolution. To enhance the throughput of OCM, we implemented a hybrid adaptive optics (hyAO) approach that combines computational adaptive optics with an intentionally aberrated imaging beam generated via hardware adaptive optics. Using hyAO, we demonstrate the depth-equalized illumination and collection ability of an astigmatic beam compared to a Gaussian beam for cellular-resolution imaging. With this advantage, we achieved volumetric OCM with a higher space-bandwidth-time product compared to Gaussian-beam acquisition that employed focus-scanning across depth. HyAO was also used to perform volumetric time-lapse OCM imaging of cellular dynamics over a 1mm x 1mm x 1mm field-of-view with 2 mu m isotropic spatial resolution and 3-minute temporal resolution. As hyAO is compatible with both spectral-domain and swept-source beam-scanning OCM systems, significant further improvements in absolute volumetric throughput are possible by use of ultrahigh-speed swept sources. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.