Chromatin remodeler Ino80C acts independently of H2A.Z to evict promoter nucleosomes and stimulate transcription of highly expressed genes in yeast.
Chromatin remodeler Ino80C acts independently of H2A.Z to evict promoter nucleosomes and stimulate transcription of highly expressed genes in yeast.
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染色质重塑剂 Ino80C 独立于 H2A.Z 发挥作用，驱逐启动子核小体并刺激酵母中高表达基因的转录。
DOI：
10.1093/nar/gkaa571
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发表时间：
2020
影响因子：
14.9
通讯作者：
Hinnebusch,AlanG
中科院分区：
文献类型：
--
作者：
Qiu,Hongfang;Biernat,Emily;Govind,ChhabiK;Rawal,Yashpal;Chereji,RăzvanV;Clark,DavidJ;Hinnebusch,AlanG
染色质重构体SWI/SnF和RSC在清除高表达酵母基因的启动子核小体方面发挥作用，特别是那些被转录因子Gcn4激活的基因。INO80重塑复合体(INO80C)可以在重组染色质中建立核小体耗尽区(−)，并参与去除组蛋白变异体H_2A.Z，使其位于NDR两侧的+1核小体上；然而，INO_80C在体内转录激活中的作用尚不清楚。分析GCN4诱导的基因inino80Δ突变的队列，揭示了Ino80Con与SWI/snf在驱逐启动子核小体和转录激活方面的作用。与SWI/Snf相比，Ino80C的功能区域更广，包括−1和+1核小体、NDR和近端基因核小体，其功能高度依赖于其功能。INO80Δ细胞的核小体清除缺陷经常伴随着TBP启动子占有率的减少和转录的减少；INO80富含在需要其重塑活性的基因上。重要的是，INO80的核耗尽损害了启动子核小体的驱逐，即使在缺乏H2A.Z的突变体中也是如此。因此，Ino80C与RSC和SWI/SNF一起在酵母基因组中广泛地作用于驱逐启动子核小体和促进转录，所有这些都至少部分地不依赖于H2A.Z的编辑。
The chromatin remodelers SWI/SNF and RSC function in evicting promoter nucleosomes at highly expressed yeast genes, particularly those activated by transcription factor Gcn4. Ino80 remodeling complex (Ino80C) can establish nucleosome-depleted regions (NDRs) in reconstituted chromatin, and was implicated in removing histone variant H2A.Z from the −1 and +1 nucleosomes flanking NDRs; however, Ino80C’s function in transcriptional activationin vivois not well understood. Analyzing the cohort of Gcn4-induced genes inino80Δmutants has uncovered a role for Ino80Con parwith SWI/SNF in evicting promoter nucleosomes and transcriptional activation. Compared to SWI/SNF, Ino80C generally functions over a wider region, spanning the −1 and +1 nucleosomes, NDR and proximal genic nucleosomes, at genes highly dependent on its function. Defects in nucleosome eviction inino80Δcells are frequently accompanied by reduced promoter occupancies of TBP, and diminished transcription; and Ino80 is enriched at genes requiring its remodeler activity. Importantly, nuclear depletion of Ino80 impairs promoter nucleosome eviction even in a mutant lacking H2A.Z. Thus, Ino80C acts widely in the yeast genome together with RSC and SWI/SNF in evicting promoter nucleosomes and enhancing transcription, all in a manner at least partly independent of H2A.Z editing.