Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice
Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice
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Omega-3 多不饱和脂肪酸可减轻成年小鼠睾丸中硫化氢引起的血睾屏障破坏
DOI：
10.1016/j.reprotox.2020.10.007
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发表时间：
2020-12-01
影响因子：
3.3
通讯作者：
Yao, Bing
中科院分区：
文献类型：
--
作者：
Wang, Shuxian;Chen, Qiwei;Yao, Bing
硫化氢(H_2S)是一种气态的细胞内信号转导分子，参与多种生理和病理条件，包括生殖条件，并干扰精子发生。血-睾丸屏障(BTB)在精子发生中起重要作用。然而，H_2S对BTB的影响及其潜在机制尚不清楚。在这里，我们研究了硫化氢和omega-3多不饱和脂肪酸(omega-3 PUFAs)对BTB和睾丸功能的影响。ICR雄性小鼠随机分为对照组、H_2S暴露组和H_2S暴露加omega-3多不饱和脂肪酸干预组。精子参数(精子密度和精子活动率)在硫化氢组下降，在omega-3干预组改善。H_2S严重破坏了BTB的完整性，BTB相关基因(ZO-1、occludin、Claudin 11)水平下降；补充omega-3可以通过上调BTB相关基因来缓解BTB的破坏，而TM4 Sertoli细胞在体外也有类似的趋势。Na2S处理组p38MAPK磷酸化水平上调，omega-3联合处理组p38MAPK磷酸化水平下调。这些发现表明，H_2S可以损害BTB，补充omega-3多不饱和脂肪酸可以减轻H_2S对男性生殖系统的毒性。我们的研究阐明了气体递质(H_2S)与BTB的关系，并确定了omega-3多不饱和脂肪酸的潜在治疗作用。
Hydrogen sulfide (H2S), a gaseous intracellular signal transducer, participates in multiple physiological and pathological conditions, including reproductive conditions, and disrupts spermatogenesis. The blood-testis barrier (BTB) plays a vital role in spermatogenesis. However, the effect of H2S on the BTB and the underlying mechanism remain unclear. Herein, we examined the effect of H2S and omega-3 polyunsaturated fatty acids (omega-3 PUFAs) on the BTB and testicular functions. ICR male mice were randomly divided into the following groups: control, H2S exposure, and H2S exposure with omega-3 PUFAs intervention. The sperm parameters (sperm concentration and sperm motility) declined in the H2S group and improved in the omega-3 intervention group. BTB integrity was severely disrupted by H2S, and the BTB-related gene levels (ZO-1, Occludin, Claudin 11) decreased; omega-3 supplementation could alleviate BTB disruption by upregulating BTB-related genes, and TM4 Sertoli cells had a similar trend in vitro. p38 MAPK phosphorylation was upregulated in the Na2S treatment group and downregulated after omega-3 cotreatment. These findings suggest that H2S can impair the BTB and that omega-3 PUFAs supplementation can attenuate H2S toxicity in the male reproductive system. Our study elucidated the relationship between a gasotransmitter (H2S) and the BTB and identified the potential therapeutic effect of omega-3 PUFAs.