Effect of methyl jasmonate on secondary metabolites of sweet basil (Ocimum basilicum L.)
Effect of methyl jasmonate on secondary metabolites of sweet basil (Ocimum basilicum L.)
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DOI：
10.1021/jf051979g
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发表时间：
2006-03-22
影响因子：
6.1
通讯作者：
Rajapakse, NC
中科院分区：
文献类型：
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作者：
Kim, HJ;Chen, F;Rajapakse, NC
将茉莉酸甲酯 (MeJA) 喷洒在健康罗勒植物上后，评估了茉莉酸甲酯 (MeJA) 在诱导甜罗勒 (Ocimum basilicum L.) 中固有生物活性化学物质方面的作用。与未进行 MeJA 的对照相比，0.1 和 0.5 mM MeJA 处理后甜罗勒的总酚含量显着增加。迷迭香酸（RA）和咖啡酸（CA）这两种酚类化合物被鉴定为甜罗勒的强抗氧化成分。 MeJA 治疗后，它们的数量也显着增加。此外，0.5 mM MeJA 处理后，丁子香酚和芳樟醇分别增加了 56% 和 43%。由于RA、CA和丁子香酚的积累，它们具有很强的2,2-二苯基-1-三硝苯肼（DPPH中心点）自由基清除活性，因此在0.5 mM MeJA处理后，甜罗勒提取物的抗氧化活性比对照高2.3倍。在DPPH中心点测定中，RA、CA和丁子香酚的EC50值分别确定为23、46和59μM，这表明它们的效率比BHT（140μM）高6、3和2.4倍。此外，0.5 mM MeJA 处理后，甜罗勒甲醇提取物中未鉴定的 HPLC 峰比对照高 4.3 倍。
The effect of methyl jasmonate (MeJA) in terms of its induction of inherent bioactive chemicals in sweet basil (Ocimum basilicum L.) was evaluated after MeJA was sprayed on healthy basil plants. The total phenolic content of the sweet basil significantly increased after 0.1 and 0.5 mM MeJA treatments compared with the control not subjected to MeJA. Two phenolic compounds, rosmarinic acid (RA) and caffeic acid (CA), were identified as strong antioxidant constituents of the sweet basil. Their amounts also significantly increased after the MeJA treatment. In addition, eugenol and linalool increased 56 and 43%, respectively, by the 0.5 mM MeJA treatment. Due to the accumulation of RA, CA, and eugenol, which possess strong 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) free radical scavenging activities, the antioxidant activity of the sweet basil extract was 2.3-fold greater than that of the control after the 0.5 mM MeJA treatment. In the DPPH center dot assay, the EC50 values of RA, CA, and eugenol were determined as 23, 46, and 59 mu M, respectively, which indicated they were 6-, 3-, and 2.4-fold more efficient than BHT (140 mu M). Besides, an unidentified HPLC peak in the methanolic extract of the sweet basil was 4.3-fold higher than that of the control after the 0.5 mM MeJA treatment.