Evaluation of ELISA for the analysis of imidacloprid in biological matrices: Cross-reactivities, matrix interferences, and comparison to LC-MS/MS
Evaluation of ELISA for the analysis of imidacloprid in biological matrices: Cross-reactivities, matrix interferences, and comparison to LC-MS/MS
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DOI：
10.1016/j.chemosphere.2021.131746
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发表时间：
2021-08-14
期刊：
影响因子：
8.8
通讯作者：
Hladik, Michelle L.
中科院分区：
文献类型：
--
作者：
Gross, Michael S.;Woodward, Emily E.;Hladik, Michelle L.
吡虫啉是全世界使用最多的农药之一，对非目标生物存在毒性问题。准确和灵敏的方法是必要的，以定量生物基质中的吡虫啉浓度，以更好地了解其命运和影响。在这里，我们评估了酶联免疫吸附测定（ELISA）试剂盒分析生物样品中的吡虫啉。以下剂量的日本鹌鹑（Coturnix japonica）与吡虫啉处理的小麦种子，血浆，肝脏和粪便样品进行了分析，通过ELISA和比较，采用液相色谱-串联质谱法（LC-MS/MS）以前的分析。测试了吡虫啉代谢物5-OH-吡虫啉、吡虫啉-烯烃、吡虫啉-尿素、去硝基吡虫啉和6-氯烟酸与商业吡虫啉ELISA试剂盒中抗体的交叉反应性。两种主要代谢产物5-OH-吡虫啉和吡虫啉-烯烃的交叉反应率为0.93- 26%。ELISA和LC-MS/MS结果呈正相关，但有较差的协议浓度：血浆和粪便中的吡虫啉浓度较高的ELISA，而肝脏中的吡虫啉浓度较高的LC-MS/MS。基质干扰分析中观察到的矩阵匹配的校准曲线的应用最小化。ELISA为这些生物基质中的吡虫啉提供了有效的筛选工具，但交叉反应物的存在混淆了结果。通过更具选择性的技术（例如，LC-MS/MS）。
Imidacloprid is among the most used pesticides worldwide and there are toxicity concerns for nontarget organisms. Accurate and sensitive methods are necessary to quantitate imidacloprid concentrations in biological matrices to better understand their fate and effects. Here we evaluated an enzyme-linked immunosorbent assay (ELISA) kit for the analysis of imidacloprid in biological samples. Following the dosing of Japanese quail (Coturnix japonica) with imidacloprid-treated wheat seeds, plasma, liver, and fecal matter samples were analyzed by ELISA and compared to previous analyses that employed liquid chromatography-tandem mass spectrometry (LC-MS/MS). Imidacloprid metabolites-5-OH-imidacloprid, imidacloprid-olefin, imidacloprid-urea, desnitroimidacloprid, and 6-chloronicotinic acid-were tested for their cross-reactivity to antibodies within the commercial imidacloprid ELISA kit. The two major metabolites, 5-OH-imidacloprid and imidacloprid-olefin, showed cross-reactivities of 0.93-26 %. ELISA and LC-MS/MS results were positively correlated but there was poor agreement in concentrations: plasma and fecal matter imidacloprid concentrations were higher by ELISA, whereas liver imidacloprid concentrations were higher by LC-MS/MS. Matrix interferences observed in analyses were minimized by the application of matrix-matched calibration curves. ELISA provided an effective screening tool for imidacloprid in these biological matrices, but the presence of cross-reactants confounded results. Confirmation of ELISA results by more selective techniques (e.g., LC-MS/MS) is suggested for complex samples.